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Mapping the role of cofactors in the conformation of proteins using spectroscopy coupled with ion mobility mass spectrometry -summary to follow

使用光谱学与离子淌度质谱联用绘制辅助因子在蛋白质构象中的作用 - 总结如下

基本信息

批准号：
1866858
负责人：
金额：
--
依托单位：
University of Manchester
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1866858
关键词：
Mapping role cofactors conformation proteins

项目摘要

We obtained funding to adapt a commercially available Synapt G2S HD MS (BBSRC BB/L002655/1) in collaboration with Waters Corp. The heart of this instrument is the ion mobility cell, IM-MS measurements are possible in this instrument, as for any Synapt mass spectrometer, but uniquely we are able to trap ions that have been conformer selected, to allow optical measurements in a so called 'photo SRIG' (stacked ring ion guide). Such optical measurements can take several forms, including IR-spectroscopy, UV/Vis-spectroscopy, which will provide structural analysis of co-factors and coenzymes, photo-dissociation to sequence the proteins as well as fluorescence to probe global conformations. Molecular mechanics calculations will generate structures to compare with those determined experimentally. This student will use this novel instrument to examine the role of cofactors on the conformations of proteins, by making measurements on the cofactor alone, the apo and the holo form of the protein. The student will be trained in biological mass spectrometry, in optical methods and in molecular mechanics. They will spend a secondment at Waters developing software to best interpret the data from this instrument. This cutting edge interdisciplinary science program is world leading and will train this student with a set of skills highly desirable both in academia and in industry.

我们获得了资金，以适应商业上可用的Synapt G2 S HD MS（BBSRC BB/L002655/1）与沃茨公司合作。该仪器的核心是离子迁移池，IM-MS测量在该仪器中是可能的，就像任何Synapt质谱仪一样，但独特的是，我们能够捕获已被构象选择的离子，以允许在所谓的“光SRIG”（堆叠环离子导向器）中进行光学测量。这种光学测量可以采取几种形式，包括IR光谱，UV/Vis光谱，其将提供辅因子和辅酶的结构分析，光解离以测序蛋白质以及荧光以探测全局构象。分子力学计算将产生结构，与实验确定的结构进行比较。这位学生将使用这种新的仪器来检查辅因子对蛋白质构象的作用，通过单独测量辅因子，载脂蛋白和蛋白质的全息形式。学生将接受生物质谱、光学方法和分子力学方面的培训。他们将在沃茨公司借调一段时间，开发软件，以最好地解释该仪器的数据。这个尖端的跨学科科学计划是世界领先的，将培养这名学生在学术界和工业界都非常理想的一套技能。