MICROENVIRONMENTS OF ENOLASE SUPERFAMILY: PROTEIN STRUCTURE

烯醇酶超家族的微环境：蛋白质结构

• 批准号: 6456777
• 负责人: D REY BANATAO
• 金额: $ 27.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6456777
• 关键词: animal tissue; bacteria; biomedical resource; drug adverse effect; intestines; lipids

We are using the FEATURE software, in collaboration with Russ Altman at Stanford University, on the enolase superfamily to characterize special features within this unique class of proteins which share a common fold, the alpha/beta or TIM barrel. The FEATURE package creates descriptions of protein microenvironments using physical and chemical properties at multiple levles of detail including features at the atomic, chemical group, residue, and secondary structural levels in order to recognize sites in three dimensional protein structures. We are performing a comparative analysis of distantly related proteins at the tertiary structural level. We are using molecular modeling tools to determine the variable and conserved elements of the members of the enolase superfamily from comparisons and superposition of structures. From these data ,we will develop a structural template for the superfamily that will allow us to differentiate the structural elements that mediate common function from those that give rise to specificity. Such templates can then be used for many purposes such as re-engineering enzymes.

我们正在与Russ合作使用功能软件 斯坦福大学的奥特曼（Altman），烯醇酶超家族 表征这一独特的蛋白质中的特殊功能 共享一个共同的折叠，α/beta或蒂姆桶。 功能 包装使用使用蛋白质微环境的描述 多个细节的物理和化学特性 包括原子，化学组，残留物和 次要结构水平以识别三个位置 尺寸蛋白质结构。 我们正在进行比较 分析三级结构上远距离相关的蛋白质 等级。 我们正在使用分子建模工具来确定 烯醇酶成员的可变和保守元素 超家族从结构的比较和叠加中。 从 这些数据，我们将为超家族开发一个结构模板 这将使我们能够区分 从引起特异性的人中调解共同的功能。 然后可以将这样的模板用于许多目的 重新设计酶。