REGULATION OF DBL RELATED ACTIVATORS OF RHO FUNCTION

DBL相关RHO功能激活剂的调控

• 批准号: 6341921
• 负责人: DANIEL L BROEK
• 金额: $ 31.78万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6341921
• 关键词: 3T3 cells; SDS polyacrylamide gel electrophoresis; autoradiography; biological signal transduction; cell growth regulation; enzyme substrate; gene expression; genetic mapping; genetic regulation; genetically modified animals; growth factor receptors; guanine nucleotide binding protein; guanine nucleotide exchange factors; guanosinetriphosphatases; in situ hybridization; laboratory mouse; laboratory rabbit; nucleic acid probes; nucleic acid sequence; phosphatidylinositols; platelet derived growth factor; polymerase chain reaction; posttranslational modifications; protein structure function; protooncogene; western blottings

DESCRIPTION: The overall goal of this application is to establish the mechanisms controlling the activity of Dbl-related guanine nucleotide exchange factors, a highly conserved family of GEFs specific for Rho GTPases. The investigator will utilize biochemical and genetic approaches to attain the following five specific aims: 1) To determine whether Src-related kinases can phosphorylate and modulate GEF activity of Dbl-related molecules. 2) To examine the functional role of PH domains of Dbl-related GEFs. 3) To investigate the significance of the interaction of Vav, a Dbl-related GEF, with components of the Ras signal transduction pathways. 4) To examine the structural basis underlying the specificity of recognition of Rho GTPases by Dbl-related GEFs. 5) To identify residues in Dbl-related GEFs which are critical for interaction with Rho GTPases.

描述：本应用程序的总体目标是建立 Dbl相关鸟嘌呤核苷酸交换活性的调控机制 这些因子是高度保守的Rho GTP酶特异性GEF家族。的 研究人员将利用生物化学和遗传学方法来实现 以下五个具体目标：1）确定Src相关激酶是否可以 磷酸化和调节Dbl相关分子GEF活性。2)审查 Dbl相关GEF的PH结构域的功能作用。3)探讨 与DBL相关的全环基金Vav与以下组成部分相互作用的重要性： Ras信号转导通路。4)检查结构基础 这是Dbl相关GEF识别Rho GTP酶的特异性的基础。 5)鉴定Dbl相关GEF中对相互作用至关重要的残基 Rho GTP酶