REGULATION OF DBL RELATED ACTIVATORS OF RHO FUNCTION

描述：此应用程序的总体目标是建立 DBL相关鸟嘌呤核苷酸交换活性的调控机制 因子，Rho GTP酶的高度保守的GEF家族。这个 研究人员将利用生化和遗传方法来实现 以下五个具体目标：1)确定Src相关激酶是否可以 磷酸化和调节DBL相关分子的全球环境基金活性。2)审查 DBL相关GEF的PH结构域的功能作用3)调查 与DBL有关的全环基金Vav与下列组成部分相互作用的意义 RAS信号转导通路。4)检视结构性基础 DBL相关GEF识别Rho GTP酶的特异性。 5)确定与DBL相关的GEF中对相互作用至关重要的残基 Rho GTP酶。

DESCRIPTION: The overall goal of this application is to establish the mechanisms controlling the activity of Dbl-related guanine nucleotide exchange factors, a highly conserved family of GEFs specific for Rho GTPases. The investigator will utilize biochemical and genetic approaches to attain the following five specific aims: 1) To determine whether Src-related kinases can phosphorylate and modulate GEF activity of Dbl-related molecules. 2) To examine the functional role of PH domains of Dbl-related GEFs. 3) To investigate the significance of the interaction of Vav, a Dbl-related GEF, with components of the Ras signal transduction pathways. 4) To examine the structural basis underlying the specificity of recognition of Rho GTPases by Dbl-related GEFs. 5) To identify residues in Dbl-related GEFs which are critical for interaction with Rho GTPases.