REGULATION OF DBL RELATED ACTIVATORS OF RHO FUNCTION

DBL相关RHO功能激活剂的调控

• 批准号: 6626587
• 负责人: DANIEL L BROEK
• 金额: $ 33.63万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626587
• 关键词: 3T3 cells; SDS polyacrylamide gel electrophoresis; autoradiography; biological signal transduction; cell growth regulation; enzyme substrate; gene expression; genetic mapping; genetic regulation; genetically modified animals; growth factor receptors; guanine nucleotide binding protein; guanine nucleotide exchange factors; guanosinetriphosphatases; in situ hybridization; laboratory mouse; laboratory rabbit; nucleic acid probes; nucleic acid sequence; phosphatidylinositols; platelet derived growth factor; polymerase chain reaction; posttranslational modifications; protein structure function; protooncogene; western blottings

DESCRIPTION: The overall goal of this application is to establish the mechanisms controlling the activity of Dbl-related guanine nucleotide exchange factors, a highly conserved family of GEFs specific for Rho GTPases. The investigator will utilize biochemical and genetic approaches to attain the following five specific aims: 1) To determine whether Src-related kinases can phosphorylate and modulate GEF activity of Dbl-related molecules. 2) To examine the functional role of PH domains of Dbl-related GEFs. 3) To investigate the significance of the interaction of Vav, a Dbl-related GEF, with components of the Ras signal transduction pathways. 4) To examine the structural basis underlying the specificity of recognition of Rho GTPases by Dbl-related GEFs. 5) To identify residues in Dbl-related GEFs which are critical for interaction with Rho GTPases.

描述：此应用程序的总体目标是建立

项目成果

In vitro, Vav is a regulated guanine nucleotide dissociation inhibitor for Ras.

在体外，Vav 是 Ras 的受调节鸟嘌呤核苷酸解离抑制剂。

• DOI: 10.1016/s0165-2478(01)00309-1
• 发表时间: 2002
• 期刊: Immunology letters
• 影响因子: 4.4
• 作者: Han,Jaewon;Das,Balaka;Broek,Daniel
• 通讯作者: Broek,Daniel

Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors.

鉴定对于与鸟嘌呤核苷酸交换因子的功能相互作用至关重要的 H-ras 蛋白残基。

• DOI: 10.1128/mcb.14.2.1104-1112.1994
• 发表时间: 1994
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Mosteller,RD;Han,J;Broek,D
• 通讯作者: Broek,D

The catalytic domain of the mouse sos1 gene product activates Ras proteins in vivo and in vitro.

小鼠 sos1 基因产物的催化结构域在体内和体外激活 Ras 蛋白。

• 发表时间: 1993
• 期刊: Oncogene
• 影响因子: 8
• 作者: Liu,BX;Wei,W;Broek,D
• 通讯作者: Broek,D

Identification of a dominant-negative mutation in the yeast CDC25 guanine nucleotide exchange factor for Ras.

鉴定 Ras 酵母 CDC25 鸟嘌呤核苷酸交换因子中的显性失活突变。

• DOI: 10.1038/sj.onc.1200893
• 发表时间: 1997
• 期刊: Oncogene
• 影响因子: 8
• 作者: Park,W;Mosteller,RD;Broek,D
• 通讯作者: Broek,D

Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.

CDC25 鸟嘌呤核苷酸交换因子中的氨基酸残基对于与 Ras 的相互作用至关重要。

• DOI: 10.1128/mcb.14.12.8117-8122.1994
• 发表时间: 1994
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Park,W;Mosteller,RD;Broek,D
• 通讯作者: Broek,D