REGULATION OF DBL RELATED ACTIVATORS OF RHO FUNCTION

描述：本应用程序的总体目标是建立 Dbl相关鸟嘌呤核苷酸交换活性的调控机制 这些因子是高度保守的Rho GTP酶特异性GEF家族。的 研究人员将利用生物化学和遗传学方法来实现 以下五个具体目标：1）确定Src相关激酶是否可以 磷酸化和调节Dbl相关分子GEF活性。2)审查 Dbl相关GEF的PH结构域的功能作用。3)探讨 与DBL相关的全环基金Vav与以下组成部分相互作用的重要性： Ras信号转导通路。4)检查结构基础 这是Dbl相关GEF识别Rho GTP酶的特异性的基础。 5)鉴定Dbl相关GEF中对相互作用至关重要的残基 Rho GTP酶

DESCRIPTION: The overall goal of this application is to establish the mechanisms controlling the activity of Dbl-related guanine nucleotide exchange factors, a highly conserved family of GEFs specific for Rho GTPases. The investigator will utilize biochemical and genetic approaches to attain the following five specific aims: 1) To determine whether Src-related kinases can phosphorylate and modulate GEF activity of Dbl-related molecules. 2) To examine the functional role of PH domains of Dbl-related GEFs. 3) To investigate the significance of the interaction of Vav, a Dbl-related GEF, with components of the Ras signal transduction pathways. 4) To examine the structural basis underlying the specificity of recognition of Rho GTPases by Dbl-related GEFs. 5) To identify residues in Dbl-related GEFs which are critical for interaction with Rho GTPases.