Photoactivated metal nanoparticles for gene-targeted DNA damage

用于基因靶向 DNA 损伤的光激活金属纳米颗粒

• 批准号: 1900512
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1900512
• 关键词: Photoactivated; metal; nanoparticles; gene; targeted

In this project we will use gold nanoparticles coated with selected DNA sequences and iridium probes to test whether they are able to be specifically toxic to the DNA of cells expressing target DNA sequences. Specific uptake will be assessed using microscopy techniques and phototoxicity assessed using biochemical assays for viability, cellular ROS and DNA damage. We will design photoactivated iridium complexes to be attached to gold nanoparticles and use DNA sequences to attempt to specifically target the mutated cells. Localisation of the nanoparticles will be studied with microscopies and the effect of two-photon photoactivation of the iridium probe will be studied using cell based toxicity assays for DNA damage and oxidative stress.

在这个项目中，我们将使用包覆选定DNA序列的金纳米粒子和铱探针来测试它们是否能够对表达目标DNA序列的细胞的DNA具有特异性毒性。特异性摄取将使用显微镜技术进行评估，光毒性将使用生物化学方法评估活力、细胞ROS和DNA损伤。我们将设计光活化铱复合物，将其附着在金纳米颗粒上，并使用DNA序列来尝试特异性靶向突变细胞。纳米粒子的定位将通过显微镜进行研究，铱探针的双光子光激活效应将通过基于细胞的DNA损伤和氧化应激毒性试验进行研究。