Transformative Iron Metal Nanoparticles with Controlled Oxidation for Magnetic Particle Imaging.

用于磁粒子成像的具有受控氧化的变革性铁金属纳米粒子。

• 批准号: 10730728
• 负责人: JACQUELINE A JOHNSON
• 金额: $ 45.11万
• 依托单位: UNIVERSITY OF TENNESSEE SPACE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10730728
• 关键词: 4T1; Biodistribution; Biology; Cardiovascular system; Cell Line; Cells; Characteristics; Chemistry; Contrast Media; Development; Dimensions; Doctor of Philosophy; Elements; Environment; Exposure to; FDA approved; Human; Image; In Vitro; Internships; Iron; Label; Ligands; Macrophage; Magnetic Resonance Imaging; Magnetic nanoparticles; Magnetism; Mammalian Cell; Mammary Neoplasms; Measurement; Metals; Methods; Molecular; Mossbauer Spectroscopy; Mus; Optics; Oxides; Oxygen; Performance; Phase; Polymers; Property; Research; Research Assistant; Research Project Grants; Resistance; Resolution; Roentgen Rays; Signal Transduction; Specificity; Structure; Students; Subcellular structure; Techniques; Thinness; Time; Tissues; Toxic effect; Training; Transmission Electron Microscopy; Work; X ray diffraction analysis; biological systems; biomaterial compatibility; contrast enhanced; contrast imaging; design; ethylene glycol; experience; fabrication; imaging modality; improved; in vivo; insight; instrument; instrumentation; iron oxide; iron oxide nanoparticle; nanoparticle; novel; oxidation; particle; physical property; preservation; summer research; undergraduate student

Project Summary This study focusses on the synthesis and characterization of an entirely new class of iron nanoparticles that transition to iron oxide nanoparticles (in vivo) over a controllable period consistent with providing biocompatibility and novel function. After synthesis, the particles will be characterized to reveal their size, structure, size distribution and magnetic properties. The degree of oxidation of FeNPs will be assessed by Mössbauer spectroscopy. The in vitro biocompatibility will be determined at various extents of oxidation. The magnitude of magnetic particle imaging signal and resolution will be determined as a function of FeNP oxidation. The Specific Aims of our research include: 1) The synthesis of iron nanoparticles for biomedical applications; 2) physical characterization and magnetic property determination of the iron nanoparticles; and 3) quantitative characterization of FeNPs, biocompatibility and MP image contrast capability in mammalian cells. We will create iron nanoparticles of uniform size with coatings that allow controllable oxidation. We will then demonstrate the transformative capabilities of these materials in producing a more sensitive nanoparticle for magnetic particle imaging. The new nanoparticle performance will be compared with VivoTrax, a commercial FeOxNP. Dr. Jacqueline Johnson’s group has performed preliminary research in the development of these nanoparticles and Dr. Johnson is a leading expert on the analysis of magnetic materials using Mössbauer spectroscopy as well as many preliminary characterization methods. Dr. Giorgio’s group has extensive experience in appropriate coatings of nanoparticles and transmission electron microscopy studies. Dr. Lu is an expert in nanoparticle chemistry; Dr. Lu and graduate research assistant, Aleia Williams, have been developing synthesis methods of FeNPs since 2020. This project will form the basis of Aleia’s and Vanderbilt GRA’s PhD work, summer research for interns at UTSI and research projects for resident undergraduates at Vanderbilt, for the duration of the project. In total, this is training for 8-10 students at various levels. The group will assess imaging performance as investigated by the company Magnetic Insight, who are developing instrumentation for Magnetic Particle Imaging.

项目摘要 本研究的重点是一个全新的铁类的合成和表征 纳米颗粒在可控的时间内转变为氧化铁纳米颗粒（体内） 与提供生物相容性和新功能一致。 合成后，将对颗粒进行表征，以揭示其尺寸、结构、尺寸 分布和磁性。FeNP的氧化程度将通过以下方式评估 穆斯堡尔谱将在不同程度上确定体外生物相容性 氧化。磁粒子成像信号的幅度和分辨率将是 确定为FeNP氧化的函数。 本研究的具体目的包括：1）合成铁纳米粒子， 生物医学应用; 2）物理表征和磁性测定 和3）FeNPs的定量表征，生物相容性 和MP图像对比度能力。 我们将创造具有涂层的均匀尺寸的铁纳米颗粒， 氧化然后，我们将展示这些材料的变革能力， 产生用于磁性粒子成像的更灵敏的纳米粒子。新 纳米颗粒性能将与VivoTrax（一种商业FeOxNP）进行比较。 博士杰奎琳约翰逊的小组已经进行了初步研究， 这些纳米粒子和约翰逊博士是一个领先的专家分析磁性 材料使用穆斯堡尔谱以及许多初步表征 方法. Giorgio博士的团队在适当的涂层方面拥有丰富的经验， 纳米粒子和透射电子显微镜研究。卢博士是一位专家， 纳米粒子化学;卢博士和研究生研究助理阿莱亚威廉姆斯，一直在 从2020年开始开发FeNPs的合成方法。该项目将成为 Aleia和范德比尔特GRA的博士工作，UTSI实习生的暑期研究和研究 项目的居民本科生在范德比尔特，为项目的持续时间。总的来说， 这是对8-10名不同级别学生的培训。该小组将评估成像 Magnetic Insight公司调查的性能，该公司正在开发 磁粒子成像仪器。