ID SEQUENCE ELEMENT ABOVE POLYPURINE TRACT ESSENTIAL FOR SIV REPLICATION

紧靠其上游的一小段富T序列 多聚尿路(PPT)在猪瘟病毒前病毒基因组中高度保守 人类和猿类免疫缺陷病毒(HIV和SIV)调查 这个“U盒”是否会影响SIVmac239复制，我们分析了 病毒基因组这个区域发生变化的突变体的特性 所有突变体要么生长迟缓(长达一个月) 延迟)或未在CEMx174细胞中检测到复制 突变体确实可以检测到复制，补偿性变化 在病毒基因组中一直观察到最常见的代偿性 变化是胸腺嘧啶的获得立即上游 Ppt，但也观察到PPT长度的显著扩张 通过转染法瞬时产生的U-box突变体严重 他们产生逆转录本的能力受损 瞬时产生的突变病毒的感染性分析 这些结果显示在RNA包装或病毒组装方面没有缺陷 确定新的结构元素，这对早期的 包括逆转录出版物在内的病毒生命周期 Ilyinskii，PO和Desrosiers，一个序列元件的RC鉴定 就在多聚尿路的上游，这对 猴免疫缺陷病毒的复制EMBO J 1998；17：3766-3774

A short stretch of T-rich sequences immediately upstream of the polypurine tract (PPT) is highly conserved in the proviral genomes of human and simian immunodeficiency viruses (HIV and SIV) To investigate whether this "U-box" influences SIVmac239 replication, we analyzed the properties of mutants with changes in this region of the viral genome All mutants were either retarded in their growth (up to one month delay) or did not replicate detectably in CEMx174 cells When U-box mutants did replicate detectably, compensatory changes were consistently observed in the viral genome The most common compensatory change was the acquisition of thymidines immediately upstream of the PPT, but marked expansion in the length of the PPT was also observed U-box mutants produced transiently by transfection were severely impaired in their ability to produce reverse transcripts in infectivity assays Analysis of transiently produced mutant virus revealed no defect in RNA pack agin g or virus assembly These results identify a new structural element important for an early step in the viral life cycle that includes reverse transcription PUBLICATIONS Ilyinskii, PO and Desrosiers, RC Identification of a sequence element immediately upstream of the polypurine tract that is essential for replication of simian immunodeficiency virus EMBO J 1998; 17:3766-3774