GENOME SEQUENCING AT LAWRENCE BERKELEY NATIONAL LABORATORY

综合基因组分析计划的总体目标是注释 具有生物学感兴趣特征的基因组序列， 有效的方式。 我们设想使用各种基因发现， 序列特征识别和基因破坏方法 这是一个努力绘制果蝇“终极基因组图谱”的过程。 这 工作有四个组成部分：（1）使用插入诱变， 主要利用P-元件增强子捕获构建体，其也提供 突变基因表达模式的窗口，以进行 在后生动物生物体中进行空前规模的基因破坏实验; (2)表达序列的研究，通过表征 cDNA分子的序列和表达模式：（3）发育和 测试算法识别的计算方法， 表达序列、控制区和DNA的其他特征 序列;以及（4）开发信息学工具来支持 实验过程，并允许最新的介绍 注释的果蝇基因组序列的信息，以世界范围内 用户研究社区 后一个目标涉及与 FlyBase数据库组（W. Gelbart，PI）开发一种通用产品 被称为“果蝇百科全书”， 开发工具，定期重新分析完成的果蝇 根据我们的项目或其他项目产生的新信息， 研究人员

The overall goal of the integrated genome analysis project is to annotate the genomic sequence with features of biological interest in a cost- effective manner. We envision the use of a variety of gene-finding, sequence-feature-identification, and gene-disruption approaches in the process of striving towards an "ultimate genome map" of Drosophila. This work has four components: (1) the use of insertional mutagenesis, primarily utilizing P-element enhancer trap constructs that also provide a window on the patterns of expression of the mutated genes, to conduct a gene disruption experiment of unprecedented scale in a metazoan organism; (2) the study of expressed sequence through the characterization of the sequence and expression pattern of cDNA molecules: (3) the development and testing of computational approaches to the algorithmic identification of expressed sequences, control regions, and other features of the DNA sequence; and (4) the development of informatics tools both to support the experimental process and to allow the presentation of up-to-date information on the annotated Drosophila genomic sequence to the world-wide user research community. This latter goal involves a collaboration with the FlyBase database group (W. Gelbart, PI) to develop a common product called the 'Encyclopaedia of Drosophila' as well as the assembly or development of tools to periodically re-analyze the completed Drosophila sequence in light of new information generated by our project or other researchers.