GENOME SEQUENCING AT LAWRENCE BERKELEY NATIONAL LABORATORY

综合基因组分析项目的总体目标是注释 在成本方面具有生物学意义的特征的基因组序列- 举止得力。我们设想使用各种基因发现技术， 序列特征识别和基因破坏方法 努力绘制果蝇“终极基因组图谱”的过程。这 工作有四个组成部分：(1)插入突变的使用， 主要利用P-元件增强子陷阱构建体，该构建体还提供 关于突变基因表达模式的窗口，以进行 后生动物中前所未有规模的基因破坏实验； (2)通过表达序列的特征来研究表达序列 CDNA分子的序列和表达模式：(3)发展和 用于算法识别的计算方法的测试 DNA的表达序列、控制区和其他特征 序列；以及(4)开发信息学工具，以支持 实验过程，并允许呈现最新的 世界各地关于注释的果蝇基因组序列的信息 用户研究社区。后一个目标涉及到与 FlyBase数据库组(W.Gelbart，PI)开发通用产品 被称为‘果蝇百科全书’以及集合或 定期重新分析已完成的果蝇的工具的开发 根据我们的项目或其他项目生成的新信息进行排序 研究人员。

The overall goal of the integrated genome analysis project is to annotate the genomic sequence with features of biological interest in a cost- effective manner. We envision the use of a variety of gene-finding, sequence-feature-identification, and gene-disruption approaches in the process of striving towards an "ultimate genome map" of Drosophila. This work has four components: (1) the use of insertional mutagenesis, primarily utilizing P-element enhancer trap constructs that also provide a window on the patterns of expression of the mutated genes, to conduct a gene disruption experiment of unprecedented scale in a metazoan organism; (2) the study of expressed sequence through the characterization of the sequence and expression pattern of cDNA molecules: (3) the development and testing of computational approaches to the algorithmic identification of expressed sequences, control regions, and other features of the DNA sequence; and (4) the development of informatics tools both to support the experimental process and to allow the presentation of up-to-date information on the annotated Drosophila genomic sequence to the world-wide user research community. This latter goal involves a collaboration with the FlyBase database group (W. Gelbart, PI) to develop a common product called the 'Encyclopaedia of Drosophila' as well as the assembly or development of tools to periodically re-analyze the completed Drosophila sequence in light of new information generated by our project or other researchers.