GENOME SEQUENCING AT LAWRENCE BERKELEY NATIONAL LABORATORY

劳伦斯伯克利国家实验室的基因组测序

• 批准号: 6202138
• 负责人: Gerald M. Rubin
• 金额: $ 148.12万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-11-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6202138
• 关键词: Drosophilidae; computer assisted sequence analysis; computer program /software; genetic library; genetic mapping; genome; image processing; information dissemination; molecular biology information system; northern blottings; nucleic acid hybridization; nucleic acid sequence; polymerase chain reaction; sequence tagged sites; transposon /insertion element

The overall goal of the integrated genome analysis project is to annotate the genomic sequence with features of biological interest in a cost- effective manner. We envision the use of a variety of gene-finding, sequence-feature-identification, and gene-disruption approaches in the process of striving towards an "ultimate genome map" of Drosophila. This work has four components: (1) the use of insertional mutagenesis, primarily utilizing P-element enhancer trap constructs that also provide a window on the patterns of expression of the mutated genes, to conduct a gene disruption experiment of unprecedented scale in a metazoan organism; (2) the study of expressed sequence through the characterization of the sequence and expression pattern of cDNA molecules: (3) the development and testing of computational approaches to the algorithmic identification of expressed sequences, control regions, and other features of the DNA sequence; and (4) the development of informatics tools both to support the experimental process and to allow the presentation of up-to-date information on the annotated Drosophila genomic sequence to the world-wide user research community. This latter goal involves a collaboration with the FlyBase database group (W. Gelbart, PI) to develop a common product called the 'Encyclopaedia of Drosophila' as well as the assembly or development of tools to periodically re-analyze the completed Drosophila sequence in light of new information generated by our project or other researchers.

整合基因组分析项目的总体目标是注释 具有生物学意义特征的基因组序列 有效的方式。 我们设想使用各种基因查找、 序列特征识别和基因破坏方法 努力绘制果蝇“终极基因组图谱”的过程。 这 工作有四个组成部分：（1）使用插入诱变， 主要利用 P 元素增强子陷阱构建体，还提供 突变基因表达模式的窗口，进行 在后生动物中进行前所未有规模的基因破坏实验； (2)通过表征来研究表达序列 cDNA分子的序列和表达模式：（3）发展和 测试算法识别的计算方法 DNA 的表达序列、控制区和其他特征 顺序; (4) 开发信息学工具以支持 实验过程并允许呈现最新的 向全世界提供带注释的果蝇基因组序列的信息 用户研究社区。 后一个目标涉及与 FlyBase 数据库小组（W. Gelbart，PI）开发通用产品 被称为“果蝇百科全书”以及大会或 开发工具来定期重新分析完整的果蝇 根据我们的项目或其他项目生成的新信息进行排序 研究人员。