REGULATION OF CGMP IN THE MANDUCA SEXTA OLFACTORY SYSTEM

描述：(改编自《调查者摘要》)细胞内 Messenger cGMP对于多种功能都很重要。它由以下方式生成 鸟苷酸环化酶(GC)。GC以多种形式存在，并且 分类为受体(膜相关)或可溶性。增加 在cGMP水平上，通过激活GC被认为是重要的 对于许多不同的途径的调节与病理生理 有意义，包括偏头痛、阳萎和哮喘。CGMP也参与其中 在神经元可塑性方面，包括LTP和LTD，在 嗅觉感受器神经元和视觉信号转导等。这 应用研究建议通过以下方式研究cGMP在神经系统中的作用 环鸟苷在嗅觉系统中的调节机制研究 Sexta，一个实验上很好的模型系统，其中解剖学和 电生理学研究得很好。本提案中描述的研究将 对于认识cGMP在糖尿病发病机制中的作用具有重要意义 嗅觉系统，在神经系统中，最后在任何系统中 其中，cGMP在调节细胞通讯和功能方面发挥作用。 PI建议解决嗅觉中鸟苷酸环化酶的调节问题 作为具体目标，回答以下问题： (1)触角叶中的哪些神经元可以并且确实对NO有反应？一氧化氮 (NO)是一种有效的可溶性GC刺激物。(2)委员会的职能是什么？ 触角叶中的NO/sGC系统？这一特定目标考察了 NO/sGC对行为性或非特异性的药理作用 触角叶的电生理输出。(3)……的活动怎么样？ 新型GC，MsGC-1，在嗅觉系统中的调节作用 Manduca Sexta？MsGC-1是一种新型GC，其表达模式提示 在嗅觉cGMP的调节中起重要作用 系统。(4)Manduca的嗅觉系统中还表达了哪些GC 性爱？PI有证据表明至少还有四个GC在嗅觉中表达 系统。PI建议克隆这些人并检查他们在调解中的角色 嗅觉系统中cGMP水平。

DESCRIPTION: (Adapted from the Investigator's Abstract) The intracellular messenger cGMP is important for a wide variety of functions. It is generated by the enzyme guanylyl cyclase (GC). GCs exist in multiple forms and are classified as being either receptor (membrane associated) or soluble. Increases in the level of cGMP through the activation of GCs is thought to be important for the regulation of many different pathways with pathophysiological significance, including migraine, impotence, and asthma. cGMP is also involved in neuronal plasticity including LTP and LTD, adaptation at the level of the olfactory receptor neurons, and visual signal transduction among others. This application proposes to investigate the role of cGMP in the nervous system by examining the mechanisms of cGMP regulation in the olfactory system of Manduca sexta, an experimentally favorable model system in which the anatomy and electrophysiology are well studied. The studies described in this proposal will have significance for the understanding of the function of cGMP in the olfactory system, in the nervous system in general, and finally in any system in which cGMP plays a role in mediating cellular communication and function. The PI proposes to address the regulation of guanylyl cyclases in the olfactory system of Manduca sexta by answering the following questions as specific aims: (1) Which neurons in the antennal lobe can and do respond to NO? Nitric oxide (NO) is a potent stimulator of soluble GCs. (2) What is the function of the NO/sGC system in the antennal lobe? This specific aim examines the effects of pharmacological manipulation of the NO/sGC for either behavioral or electrophysiological outputs of the antennal lobe. (3) How is the activity of the function of the novel GC, MsGC-1, regulated in the olfactory system of Manduca sexta? MsGC-1 is a new type of GC whose expression pattern suggests that it plays an important role in the mediation of cGMP in the olfactory system. (4) What other GCs are expressed in the olfactory system of Manduca sexta? The PI has evidence of at least four more GCs expressed in the olfactory system. The PI proposes to clone these and examine their roles in mediating cGMP levels in the olfactory system.