Biotechnology-Nanotechnology Hybrid Materials for Diagnostic Devices in Health and Agriculture

用于健康和农业诊断设备的生物技术-纳米技术混合材料

• 批准号: 1924652
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1924652
• 关键词: Biotechnology; Nanotechnology; Hybrid; Materials; Diagnostic

Immunosorbent "sandwich" assays (including ELISA) that rely on fluorescence detection have become a key technology in medical testing, drug discovery, biotechnology and cellular imaging. The high selectivity of antibody binding, combined with the high sensitivity that is enabled by fluorescence spectroscopy, has led to huge advances inthe diagnostic testing of a variety of human, animal and plant diseases. Typically, these immunosorbent assays employ an immobilised antibody to capture the target biomarker from the biological sample, followed by the immobilisation of a second antibody bearing a fluorescent label.To maximise the fluorescence output from the label, many researchers have started to harness metal enhanced fluorescence (MEF), as a means to improve assays ensitivity. Here, the co-location of a metallic nanoparticle in the vicinity of the antibody results in a large plasmonic enhancement of fluorescence output (in the order of 100-fold). Indeed, the arrival of MEF assay technology in the industrial and diagnostic application context is imminent.This project aims at improving the sensitivity of these MEF-based assay systems, by the application of state-of-the-art bioconjugate chemistry to control the orientation of the immobilised antibodies with respect to the nanoparticle. It is expected that such uniform antibody orientation, with all binding domains pointing "outwards" (away from the nanoparticle) would both improve their binding capacity against very low levels of analyte, as well as maximise MEF by ensuring the optimum distance between fluorophore and nanoparticle.This project will focus on the development of expertise in assay technology , synthetic chemistry, spectroscopy and nanotechnology - underpinning skills that are critical for the biotechnology sector. The project is part-funded by Aeirtec Ltd., a diagnostics development company, and the researcher will spend time on a placement at Aeirtec's laboratories.

依赖于荧光检测的免疫吸附“夹心”测定（包括ELISA）已经成为医学测试、药物发现、生物技术和细胞成像中的关键技术。抗体结合的高选择性，结合荧光光谱法的高灵敏度，导致了各种人类，动物和植物疾病的诊断测试的巨大进步。通常，这些免疫吸附测定采用固定化抗体从生物样品中捕获目标生物标志物，然后固定带有荧光标记的第二抗体。为了最大化标记的荧光输出，许多研究人员开始利用金属增强荧光（MEF）作为提高测定灵敏度的手段。这里，金属纳米颗粒在抗体附近的共定位导致荧光输出的大的等离子体增强（大约100倍）。事实上，MEF检测技术在工业和诊断应用领域的到来迫在眉睫。该项目旨在通过应用最先进的生物缀合物化学来控制固定化抗体相对于纳米颗粒的方向，从而提高这些基于MEF的检测系统的灵敏度。预期这种均匀的抗体取向，所有结合结构域都指向“向外”，（远离纳米颗粒）将提高它们对非常低水平的分析物的结合能力，以及通过确保荧光团和纳米颗粒之间的最佳距离来最大化MEF。该项目将专注于开发检测技术，合成化学，光谱学和纳米技术-对生物技术部门至关重要的基础技能。该项目由Aeirtec有限公司提供部分资金，一家诊断开发公司，研究人员将在Aeirtec的实验室工作。