Sequencing the Dictyostelium genome

描述(申请人提供)：进化保护 蛋白质中的结构-功能关系使我们能够整合信息 并对不同物种的生物现象做出推论 不同的有机体。在接下来的十年里，对这一功能的基本理解 大多数基因都将从几种模式生物中获得。网柄网柄菌是一种 社会阿米巴，他的研究有助于我们对多样性的理解 许多真核生物共有的生物过程。对模型的深入研究 Dictyostelius等生物将告诉我们关于人类生物学和这方面的信息 将不可避免地有助于疾病的检测和治疗。 这项提案描述了一项持续努力，以对大约一半的 网柄网柄菌基因组。之前的项目阶段侧重于可行性。 6号染色体的研究和测序。这项建议针对4号染色体 和5个基因加起来约占34-Mb基因组的三分之一。我们会 使用全染色体鸟枪测序结合一种新的 最小重叠的大插入克隆集，以生成连续和 高精度的序列。组装好的DNA将与 高分辨率物理地图，将根据该地图计划完成实验 并执行以完成序列。这段序列将有丰富的注释 并提交到公共数据库。所有原始序列数据都将可用 尽快通过我们的网站。 全基因组序列的获得有望加速研究 细胞和发育生物学的基本机制及其面临的挑战 现在是定义这些基因组中包含的基因并确定它们的 功能。能够适应高通量方法的微生物 对Dictyostelius等的调查已经并将继续 在这一努力中起到了重要作用。网柄网柄菌基因组序列将提供 第一次详细地瞥见一个比 单细胞生物体，但仍然足够简单，可以完全理解 可预见的未来。基因组数据将使所有领域的专家 生物学来丰富最初的发现，并对其他 物种。从实验上容易处理的数据中获得完整的序列数据 生物，如网柄网柄菌，将继续刺激基因组 在包括人类在内的许多系统中进行调查。

DESCRIPTION (provided by applicant): Evolutionary conservation of structure-function relationships in proteins allows us to integrate information from various species and make inferences about biological phenomena in different organisms. Over the next decade a basic understanding of the function of most genes will be obtained for several model organisms. Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to many eukaryotes. The in-depth study of model organisms such as Dictyostelium will inform us about human biology and this will inevitably aid in the detection and treatment of disease. This proposal describes a continuing effort to sequencing about one-half of the Dictyostelium genome. The previous project period focused on feasibility studies and the sequencing of chromosome 6. This proposal targets chromosomes 4 and 5 which together constitute about one-third of the 34-Mb genome. We will use whole chromosome shotgun sequencing combined with sample sequencing of a minimum overlapping set of large-insert clones to generate contiguous and highly accurate sequence. The assembled DNA will be integrated with a high-resolution physical map from which finishing experiments will be planned and carried out to complete the sequence. The sequence will be richly annotated and submitted to public databases. All raw sequence data will be made available as soon as possible via our website. The availability of complete genome sequences promises to accelerate research into fundamental mechanisms of cell and developmental biology and the challenge now is to define the genes contained in those genomes and determine their function. Microorganisms that are amenable to high-throughput methods of investigation such as Dictyostelium have been and will continue to be instrumental in this endeavor. The Dictyostelium genome sequence will provide the first detailed glimpse of a biological system that is more complex than single-celled organisms, yet still simple enough to understand completely in the foreseeable future. The genomic data will allow specialists in all areas of biology to enrich the initial findings and make useful extrapolations to other species. Complete sequence data obtained from experimentally tractable organisms, such as Dictyostelium, will continue to stimulate genomic investigations in many systems, including humans.