FUNCTIONAL GENOMICS OF DICTYOSTELIUM

描述：真核生物全基因组序列的可用性 承诺加快细胞和细胞的基本机制的研究 发育生物学。在充分利用这些信息并进行研究之前 生物体的生物学从其整个基因组的背景来看，一个人必须是 能够：(1)识别基因组中的相关信息，(2) 了解信息如何转化为功能，以及(3)整合 这些数据形成了一幅全面的生物学图景。调查人员的是 开发工具，包括实验和理论，这将促进 这些目标是为了研究所有的有机体。他们打算通过以下方式测试这些工具 对土壤中的盘基网生阿米巴进行了分析，并与 与在其他基因组分析中发现的那些发现相一致。该项目将 将技术开发和遗传资源获取与 基于基因组的功能分析方法。最近发布的报告 提供充分的证据表明微阵列数据可以有效地用于 遗传分析(表型、上位性试验等)。调查人员的 基本想法是，细胞的转录状态可以用作高分辨率 表型具有必要的辨别能力，以放置所有 在一个框架调控网络内的双沟丝菌中的基因， 可以计划进行适当的实验来测试分子机制。这个 研究人员将检查所有基因在生长和生长过程中的表达 在大约5,000个基因敲除突变体中的每一个中都发育了双沟柄菌。这 数据，当与来自并行表型和其他 传统的表型鉴定将使我们能够对基因进行可测试的预测 功能，并提出监管网络。功能的独特价值 基因组学在于大型数据库的力量，能够提供广泛的、 大概是对生物系统不那么偏颇的观点。这一方法将开启 把发展的探索推向更广阔的视野。它将再次强调 整个生物体作为基本的生物单位并揭示 信号通路与细胞类型之间的关系 从对一小群基因的分析中得到了赞赏。调查人员的是 对网柄基菌生物学中所有人都共有的方面感兴趣 真核生物，这将是定义两个 单个基因的功能和调控等级的组织 它们在开发中运作。相对简单和遗传上的易操纵性 将在基因组分析中处于领先地位 在接下来的五年里，多细胞的发展。

DESCRIPTION: The availability of complete eukaryotic genome sequences promises to accelerate research into fundamental mechanisms of cell and developmental biology. Before one can fully exploit this information and study the biology of an organism from the context of its entire genome one must be able to: (1) identify the relevant information within the genome, (2) understand how the information is translated into function, and (3) integrate these data into a comprehensive biological picture. The investigators' are developing tools, both experimental and theoretical, that will facilitate these goals for the study of all organisms. They intend to test these tools by analyzing the soil amoebae Dictyostelium discoideum, and by comparing there findings to those found in the analyses of other genomes. The project will combine technology development and genetic resource acquisition with genome-based approaches to functional analysis. Recent published reports provide ample evidence that microarray data can be used effectively for genetic analysis (phenotyping, epistasis tests, etc.). The investigators' basic idea is that the transcriptional state of a cell can be used as a high-resolution phenotype with the necessary discriminating power to place all of the genes in Diclyostelium within a framework regulatory network, from which, the appropriate experiments to test molecular mechanisms can be planned. The investigators' will examine the expression of all genes during the growth and development of Diclyostelium within each of about 5,000 knockout mutants. This data, when combined with the fitness data from parallel phenotyping and other traditional phenotyping, will allow us to make testable predictions of gene function and to propose regulatory networks. The unique value of functional genomics lies in the power of large databases to provide a broad, and presumably less-biased, view of biological systems. This approach will open the exploration of development to a broader viewpoint. It will re-emphasize the entire organism as the fundamental biological unit and reveal relationships between signaling pathways and cell-types that could not be appreciated from the analyses of small groups of genes. The investigators' are interested in those aspects of Dictyostelium biology that are common to all eukaryotic organisms, and that will be informative for defining both the function of individual genes and the organization of regulatory hierarchies that operate in development. The relative simplicity and genetic tractability of organisms such as Dictyostelium will lead the way in the genomic analyses of multicellular development over the next five years.