FUNCTIONAL GENOMICS OF DICTYOSTELIUM

描述：完整的真核基因组序列的可用性 有望加速对细胞基本机制的研究 发育生物学在人们能够充分利用这些信息和研究 从生物体的整个基因组来看， 能够：（1）识别基因组内的相关信息，（2） 理解信息如何转化为功能，以及（3）整合 将这些数据转化为一幅全面的生物学图像。调查人员的任务是 开发实验和理论工具， 这些目标是为了研究所有生物体。他们打算测试这些工具， 对土壤变形虫盘基网柄藻进行了分析， 与其他基因组分析中发现的结果相比较。该项目将 联合收割机技术开发和遗传资源获取与 基于基因组的功能分析方法。最近发表的报告 提供了充分的证据表明，微阵列数据可以有效地用于 遗传分析（表型分析、上位性试验等）。调查人员的 基本思想是细胞的转录状态可以用作高分辨率的 表型与必要的辨别力，把所有的 Diclyosteroid中的基因在一个框架调控网络中，从中， 可以计划适当的实验来测试分子机制。的 研究人员将检查生长过程中所有基因的表达， 在约5，000个敲除突变体中的每一个中产生了双溶血素。这 数据，当与来自平行表型分析和其他 传统的表型分析，将使我们能够对基因进行可测试的预测， 功能，并提出监管网络。功能的独特价值 基因组学在于大型数据库的力量， 大概是更少偏见的生物系统的观点。这种方法将打开 把对发展的探索推向更广阔的视野。它将再次强调 整个有机体作为基本的生物单位， 信号通路和细胞类型之间的关系， 从对小群体基因的分析中可以看出。调查人员的任务是 对网骨藻生物学的那些共同点感兴趣 真核生物，这将是信息定义这两个 单个基因的功能和调控层次的组织 在发展中运作。相对简单和遗传易处理 对像网囊藻这样的生物的研究将在基因组分析中起主导作用 在未来五年内多细胞的发展。

DESCRIPTION: The availability of complete eukaryotic genome sequences promises to accelerate research into fundamental mechanisms of cell and developmental biology. Before one can fully exploit this information and study the biology of an organism from the context of its entire genome one must be able to: (1) identify the relevant information within the genome, (2) understand how the information is translated into function, and (3) integrate these data into a comprehensive biological picture. The investigators' are developing tools, both experimental and theoretical, that will facilitate these goals for the study of all organisms. They intend to test these tools by analyzing the soil amoebae Dictyostelium discoideum, and by comparing there findings to those found in the analyses of other genomes. The project will combine technology development and genetic resource acquisition with genome-based approaches to functional analysis. Recent published reports provide ample evidence that microarray data can be used effectively for genetic analysis (phenotyping, epistasis tests, etc.). The investigators' basic idea is that the transcriptional state of a cell can be used as a high-resolution phenotype with the necessary discriminating power to place all of the genes in Diclyostelium within a framework regulatory network, from which, the appropriate experiments to test molecular mechanisms can be planned. The investigators' will examine the expression of all genes during the growth and development of Diclyostelium within each of about 5,000 knockout mutants. This data, when combined with the fitness data from parallel phenotyping and other traditional phenotyping, will allow us to make testable predictions of gene function and to propose regulatory networks. The unique value of functional genomics lies in the power of large databases to provide a broad, and presumably less-biased, view of biological systems. This approach will open the exploration of development to a broader viewpoint. It will re-emphasize the entire organism as the fundamental biological unit and reveal relationships between signaling pathways and cell-types that could not be appreciated from the analyses of small groups of genes. The investigators' are interested in those aspects of Dictyostelium biology that are common to all eukaryotic organisms, and that will be informative for defining both the function of individual genes and the organization of regulatory hierarchies that operate in development. The relative simplicity and genetic tractability of organisms such as Dictyostelium will lead the way in the genomic analyses of multicellular development over the next five years.