PROJECT I - Analysis of Gene Function by Parallel Phenotyping

项目 I - 通过平行表型分析基因功能

• 批准号: 8252937
• 负责人: ADAM KUSPA
• 金额: $ 37.75万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-10-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8252937
• 关键词: Activities of Daily Living; Amoeba genus; Bacteria; Base Pairing; Biological Models; DNA; DNA Sequence; Data; Deposition; Development; Dictyostelium; Discrimination; Drops; Eukaryota; Eukaryotic Cell; Gene Expression Profile; Genes; Genetic; Genome; Genomics; Glean; Growth; Growth and Development function; Haploidy; Human; Immune; Immune response; Immune system; Individual; Infection; Instruction; Investigation; Laboratories; Link; Location; Measures; Mediating; Microarray Analysis; Molecular; Natural Immunity; Noise; Oligonucleotide Microarrays; Oligonucleotides; Organism; Pathway interactions; Phenotype; Physiological; Plasmids; Population; Reading; Regulation; Relative (related person); Research Personnel; Role; Sampling; Site; System; Techniques; Technology; Testing; Work; design; fitness; follow-up; functional genomics; gene function; improved; insight; interest; mutant; next generation; pressure; programs; research study; response; restriction enzyme; tool development

PROJECT SUMMARY (See instructions): This Program Project is aimed at understanding the mechanisms that control growth and multicellular development in Dictyostelium. By examining the functions of a large number of genes we will begin to formulate a global view of the regulatory networks In this organism. In the previous project period we took a functional genomics approach to high-throughput mutant phenotyping, using molecular barcodes, that has allowed us to draw functional inferences for hundreds of genes. We will revolutionize our parallel phenotyping platform using Next Generation Sequencing technologies that should yield dramatic improvements in barcode quantification so that more information can be gleaned from every new experiment. Over the next five years we will focus our efforts on understanding bacterial recognition in Dictyostelium, both during the growth of solitary amoebae and in the context of an innate immune response during development. We will define innate immune recognition of bacteria by amoebae in molecular terms by characterizing the genes and pathways involved. We will intersect the transcriptional profiling data from Project II with the physiological data provided by this project to uncover links between gene function and patterns of gene expression. The data we produce will also be used by Project 111 for extracting information about the genetic networks that coordinate bacterial recognition in Dictyostelium

项目总结(见说明)： 该计划项目旨在了解控制生长和多细胞的机制 网柄网柄菌的发育。通过研究大量基因的功能，我们将开始 对这一生物体中的调控网络形成一个全球视野。在前一个项目期间，我们采取了一个 使用分子条形码的高通量突变表型的功能基因组学方法，具有 使我们能够对数百个基因进行功能推断。我们将彻底改变我们的平行关系 使用下一代测序技术的表型平台应该会产生戏剧性的结果 改进条形码量化，以便从每个新的 做实验。在接下来的五年里，我们将集中精力了解细菌识别 在孤立阿米巴的生长过程中和在先天免疫反应的背景下 在开发过程中。我们将从分子的角度定义阿米巴对细菌的先天免疫识别 通过描述相关的基因和途径。我们会将转录特征数据从 项目II使用该项目提供的生理数据来揭示基因功能和 基因表达模式。我们产生的数据也将被Project 111用来提取信息 关于Dictyostelius中协调细菌识别的遗传网络