STRUCTURE & INHIBITOR STUDIES OF TERPENE CYCLASE ARISTOLOCHENE SYNTHASE

结构

• 批准号: 6491101
• 负责人: DAVID W CHRISTIANSON
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-15 至 2002-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6491101
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

An 8 hour shift in 1997 on beam-line A1 revealed that crystals diffracted at 4 degrees C to about 3.4 A, but they were very radiation sensitive. Methods were develop at Florida State University, to co-crystallize the virus in the presence of a cryoprotectant. Our F1 beam-line time in April 1998 established that these crystals were stable at 100 K, diffracting for about 4 hours. From 24 crystals, about 130 degrees of 3.5 A data, and 160 degrees of 4.5 A data were collected. Data are currently being processed.

1997年在光束线A1上进行的8小时移动表明， 衍射在4摄氏度到约3.4埃，但他们非常辐射 敏感 方法由佛罗里达州立大学开发， 在冷冻保护剂的存在下使病毒共结晶。 我们的F1 1998年4月的光束线时间确定，这些晶体是 在100 K下稳定，衍射约4小时。 从24块水晶中， 大约130度的3.5 A数据，和160度的4.5 A数据， 收集。 数据目前正在处理中。