X-RAY STUDIED OF REL-MTB

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们已经结晶了Relmtb，一种来自结核分枝杆菌的Rel/SPoT同源物，可以调节该细菌的严格反应。敲除该基因表明对抗生素的敏感性增加。我们收集了2.3昂分辨率的原生数据。然而，由于晶体尺寸非常小（<50 um），这些数据的总体I/sigma(I)很低（~5），Rsym相当高（0.16）。我们期望同步加速器的数据能够改善这些问题，也将允许我们通过MAD或MIRAS来解决结构问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have crystallized Relmtb, a Rel/SPoT homolog from M tuberculosis that modulates the stringent response in that bacterium. Knockouts of this gene demonstrate increased susceptibility to antibiotics. We have collected native data to 2.3 Ang resolution. However, due to the very small (<50 um) crystal size, overall I/sigma(I) for these data are low (~5) and Rsym is rather high (0.16). We expect that synchrotron data will ameliorate these problems, and will also allow us to solve the structure by MAD or MIRAS.