CRYSTAL STRUCTURE ANALYSIS OF STEROL METHYL TRANSFERASE

甾醇甲基转移酶的晶体结构分析

• 批准号: 7357719
• 负责人: DAVID W CHRISTIANSON
• 金额: $ 2.14万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357719
• 关键词: CRYSTAL; STRUCTURE; ANALYSIS; STEROL; METHYL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sterol methyl transferase (SMT) is an important enzyme that regulates carbon flow in the sterol pathway of fungi. The enzyme catalizes the reaction that transfer a methyl group from (S)-adenosyl-L-methionine to different steriols and other natural products are widespread in biochemstry.New extensive investigations have led to consider a stereochemical model of stearol methylation that describes the topological constraints imposed by sterol substrate in the binding pocket of the SMT. With this regard the crystal structure could represent the experimental fundation of this hyphotetical model and could represent the starting point for the design of new inhibitors of the enzyme. We got the unit cell parameters and a data set with resolution of 5.0 A. Recently we got crystals in different conditions. We need to do X-ray experiments at CHESS to improve the resolution of diffraction data, and to follow with a MAD experiment to solve the phase problem.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。类固醇甲基转移酶(SMT)是调节真菌类固醇途径中碳流的重要酶。该酶催化将一个甲基从(S)-腺苷-L-蛋氨酸转移到不同的甾醇和其他天然产物中的反应在生物化学中广泛存在。新的广泛的研究导致考虑硬脂醇甲基化的立体化学模型，该模型描述了甾醇底物在SMT结合口袋中施加的拓扑约束。在这一点上，晶体结构可以代表这个菌丝模型的实验基础，也可以代表设计新的酶抑制剂的起点。我们得到了晶胞参数和分辨率为5.0A的数据集。最近，我们得到了不同条件下的晶体。我们需要在CHESS上进行X射线实验，以提高衍射数据的分辨率，并随后进行MAD实验来解决位相问题。