STRUCTURE & ASSEMBLY OF RETROVIRUSES

结构

• 批准号: 6444668
• 负责人: Volker M. Vogt
• 金额: $ 29.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444668
• 关键词: Chordata; animal tissue; biomedical resource; growth /development; infection; virus

In vitro particles of Rous sarcoma virus (RSV) were assembled with Gag protein expressed in E. coli and E. coli RNA and examined in the STEM. They appeared very much like immature retroviruses and had ma ses of about 80 Md, corresponding to about 1200 Gag proteins per particle. Other constructions are being studied. In addition, several laboratories are becoming interested in measuring the masses of retroviruses since most data bn them is about 20 years old. Some preliminary results for RSV have been obtained. Two strains of (whole) in vivo virus particles were purified from infected turkey cells. One has a genorne of only Gag, Pol, and Env. The other carries an additional gene (alkaline phosphatase) making the genome -20% larger (like the "real" RSV which carries the src oncogene). The size of retroviruses appears variable and one hypothesis is that the size is determined by the amount of RNA packaged. Mass measurements from the STEM data indicate that the MWs appear about the same.

劳斯肉瘤病毒（RSV）的体外颗粒与 Gag蛋白在E. coli和E.大肠杆菌RNA，并在 茎 它们看起来非常像未成熟的逆转录病毒， 约80 Md的ses，对应于约1200 Gag蛋白/ 粒子 其他建筑正在研究中。 此外，本发明还提供了一种方法， 几个实验室开始对测量质量感兴趣 因为大多数关于逆转录病毒的数据都是20年前的。 一些 已获得RSV的初步结果。 两株 从感染的火鸡中纯化（完整）体内病毒颗粒 细胞 其中一个基因组只有Gag、Pol和Env。另一个携带 一个额外的基因（碱性磷酸酶）使基因组-20% 更大（像携带src致癌基因的“真实的”RSV）。 大小 逆转录病毒的大小似乎是可变的，一种假设是， 由包装的RNA量决定。 质量测量从 STEM数据表明MW看起来大致相同。