ELECTRON CRYSTALLOGRAPHIC STUDIES OF ACROSOMAL BUNDLE

鲎精子的顶体束可以被认为是 三维准晶体和重建使用 晶体学方法 然而，它们既不是“无限”的， 大的，大的，大的， 沿观察方向的厚度为沿着的晶胞。 而且因为 它们的形状、束通常不具有优选的方位角 方向，这对方向估计提出了挑战， 精致。 我们制定了一个记录和处理 电子冷冻显微镜图像，不同于经典的 二维晶体重建技术。 这些 发展使我们能够合并断层倾斜系列的数据， 冰包顶体束。 我们的目标是准确地确定 衍射最大值处的振幅和相位（以hkl表示） 折射率，并从衍射计算三维图 数据 我们在这个项目中的研究工作不仅导致了 新的结构信息的束，但也导致了一个 在实验方面加强我们的研究基础设施 和计算能力。

Acrosomal bundles from Limulus sperm can be considered as three-dimensional quasi-crystals and reconstructed using a crystallographic approach. However, they are neither "infinitely" large with respect to the borders of the bundle, nor are they a single unit cell in thickness along the viewing direction. Also, because of their shape, bundles do not generally have a preferred azimuthal orientation, which poses challenges for orientation estimation and refinement. We have developed a strategy for recording and processing electron cryo-microscopic images that differs from classical two-dimensional crystalline reconstruction techniques. These developments allowed us to merge data from tomographic tilt series of ice-embedded acrosomal bundles. The goal is to accurately determine amplitudes and phases at the diffraction maxima in terms of hkl indices, and compute a three-dimensional map from the diffraction data. Our research effort in this project has resulted not only in novel structural information about the bundle but also led to an enhancement of our research infrastructure in terms of experimental and computational capabilities.