"SINGLE PARTICLE" CRYO-ET

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一核心项目的目的是建立一个实验和计算程序，通过使用电子冷冻层析成像(CRYO-ET)解决结构问题，然后进行层析后分类、排列和平均。低温冷冻技术是近年来出现的一种弥合原子性结构和完整细胞之间的鸿沟的强大技术。具体地说，最近提出的执行所谓“单粒子”低温实验的想法与NCMI的现有专业知识密切相关，我们相信，通过利用我们在高分辨率数据收集和处理方面的经验，我们可以在这一领域做出贡献。层析成像可以帮助解决上述提出的多构象状态的问题，因为它具有提供关于大分子组装的三维信息的独特能力，极大地减少了结构异质性与取向的混淆相互作用，而这种相互作用往往很难用每个粒子的单一2D图像来区分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core project is aimed at setting up an experimental and computational procedure to address structural questions by using electron cryo-tomography (cryo-ET) followed by post-tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells. Specifically, the recent idea to perform so called "single particle" cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing interaction of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.