"SINGLE PARTICLE" CRYO-ET

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 这个核心项目的目的是建立一个实验和计算程序，以解决结构问题，通过使用电子冷冻断层扫描（冷冻ET），然后进行断层扫描后的分类，对齐和平均。近年来，Cryo-ET已成为一种强大的技术，用于弥合原子结构和整个细胞之间的差距。具体来说，最近执行所谓的“单粒子”冷冻ET的想法与NCMI现有的专业知识密切相关，我们相信我们可以通过利用我们在高分辨率数据收集和处理方面的经验在这一领域做出贡献。断层扫描可以帮助解决以上提出的多个构象状态的问题，因为它具有提供关于大分子组装体的三维信息的独特能力，大大减少了结构异质性与取向的混乱相互作用，这种相互作用通常难以用每个颗粒的单个2D图像来区分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core project is aimed at setting up an experimental and computational procedure to address structural questions by using electron cryo-tomography (cryo-ET) followed by post-tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells. Specifically, the recent idea to perform so called "single particle" cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing interaction of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.