SINGLE PARTICLE CRYO--ET

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个核心项目的目的是建立一个实验和计算程序来 通过使用电子冷冻断层扫描(CRYO-ET)解决结构问题，然后进行后扫描 层析分类、对齐和平均。冷冻机是近年来出现的一种 一种强大的技术，可以弥合原子式结构和整个细胞之间的差距。 具体地说，最近提出的执行所谓的单粒子低温实验的想法与 NCMI现有的专业知识，我们相信我们可以在这一领域做出贡献 通过利用我们在高分辨率数据收集和处理方面的经验。 层析成像可以帮助解决上述提出的多构象状态的问题 因为它独特的能力提供了关于大分子的三维信息 组装，极大地减少了结构异质性与 通常很难用每个粒子的单个2D图像来区分的方向。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core project is aimed at setting up an experimental and computational procedure to address structural questions by using electron cryo-tomography (cryo-ET) followed by post- tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells. Specifically, the recent idea to perform so called 'single particle' cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing interaction of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.