SINGLE PARTICLE CRYO--ET

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这个核心项目的目的是建立一个实验和计算程序， 通过使用电子冷冻断层扫描（cryo-ET）解决结构问题，然后进行后处理， 层析成像分类、对准和平均。Cryo-ET近年来出现， 这是一种在原子结构和整个细胞之间弥合差距的强大技术。 具体地说，最近的想法，执行所谓的'单粒子'冷冻ET是密切相关的， NCMI现有的专业知识，我们相信我们可以在这一领域做出贡献 利用我们在高分辨率数据收集和处理方面的经验。 断层扫描可以帮助解决上述多个构象状态的问题 因为它能提供大分子的三维信息 组装，大大减少了结构异质性与 通常难以用每个颗粒的单个2D图像来区分这些取向。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core project is aimed at setting up an experimental and computational procedure to address structural questions by using electron cryo-tomography (cryo-ET) followed by post- tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells. Specifically, the recent idea to perform so called 'single particle' cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing interaction of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.