CORE--IMMUNOLOGY FACILITY

核心——免疫设施

• 批准号: 6506206
• 负责人: DOROTHY E. LEWIS
• 金额: $ 12.95万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6506206
• 关键词: AIDS; biomedical facility; clinical research; confocal scanning microscopy; cytokine; cytotoxic T lymphocyte; flow cytometry; human immunodeficiency virus; human tissue; immunologic assay /test; immunology; training

The CFAR 1rnmunology Core is designed to provide both state-of-the-art flow cytometric capabilities as well as expertise and technical assistance for numerous immunological assays desired by members of the CFAR. The core is dedicated to providing such service in a timely, cost-effective, and efficient manner. The core is headed by Dr. Dorothy E. Lewis, who has over 20 years of flow and immunologic experience and a staff of well- trained personnel. The core consists of two flow cytometers, an analytical one and a high speed cell sorter, a BL-2 containment room for immunological assays, and a confocal microscope. Cytokine measurement expertise is provided by Dr. Reuben at M.D. Anderson Cancer Center, located across the street from Baylor College of Medicine, on a fee-for- service basis. In the previous funding period, the core has been essential for studies involving immune function in pediatric HW and has facilitated interactions with clinical and basic science researchers. In particular, the core was key for studies of Tat kinase activity in primary lymphocytes, in detection of cytokine messages, and in analysis of CD8+ T cell function in HWinfected children. The core has also developed new assays for the users, including improved HIV-speciflc cytotoxic T lymphocyte assays and cytokine quantitation in tissues. A range of services is provided by this core which include flow cytometry, lymphocyte function assays and confocal microscopy. Training on the use of instrumentation and data interpretation is also a key component of the core. The core will be overseen by a committee. The Core Director will have responsibility for day-to-day running of the core, and priorities for usage are prescribed in a standardized manner mcluding an appropriate charge-back system. It is anticipated that at least 19 investigators at Baylor College of Medicine and at The University of Texas, UTMB-Galveston, Texas A&M University, and Tulane University School of Medicine will benefit. ~

CFAR 1rnmunology Core 旨在为 CFAR 成员所需的众多免疫学测定提供最先进的流式细胞术功能以及专业知识和技术援助。核心致力于及时、经济、高效地提供此类服务。该核心由 Dorothy E. Lewis 博士领导，她拥有 20 多年的流程和免疫学经验以及一批训练有素的人员。其核心由两台流式细胞仪、一台分析仪和一台高速细胞分选仪、一间用于免疫分析的 BL-2 隔离室以及一台共聚焦显微镜组成。细胞因子测量专业知识由位于贝勒医学院街对面的 M.D. Anderson 癌症中心的 Reuben 博士提供，按服务收费。在之前的资助期间，该核心对于涉及儿科 HW 免疫功能的研究至关重要，并促进了与临床和基础科学研究人员的互动。特别是，该核心对于研究原代淋巴细胞中的 Tat 激酶活性、检测细胞因子信息以及分析 HWin 感染儿童的 CD8+ T 细胞功能至关重要。该核心还为用户开发了新的检测方法，包括改进的 HIV 特异性细胞毒性 T 淋巴细胞检测和组织中细胞因子定量。该核心提供一系列服务，包括流式细胞术、淋巴细胞功能测定和共聚焦显微镜。仪器使用和数据解释培训也是核心的关键组成部分。核心将由一个委员会监督。 核心主管将负责核心的日常运行，并以标准化方式规定使用优先级，包括适当的退款系统。预计贝勒医学院、德克萨斯大学、UTMB-加尔维斯顿分校、德克萨斯农工大学和杜兰大学医学院的至少 19 名研究人员将受益。 ～