CORE--IMMUNOLOGY FACILITY

CFAR 1免疫学核心旨在提供最先进的流式细胞术功能，以及CFAR成员所需的众多免疫学分析的专业知识和技术援助。核心致力于以及时、经济、高效的方式提供此类服务。核心是由多萝西·e·刘易斯博士，谁拥有超过20年的流动和免疫经验和工作人员训练有素的人员。核心包括两台流式细胞仪，一台分析仪和一台高速细胞分选仪，一个用于免疫测定的bl2密闭室和一台共聚焦显微镜。细胞因子测量专业知识是由M.D.安德森癌症中心的Reuben博士提供的，该中心位于贝勒医学院对面，是收费服务的基础。在之前的资助期内，核心对涉及儿童HW免疫功能的研究至关重要，并促进了与临床和基础科学研究人员的互动。特别是，核心是研究原发性淋巴细胞中Tat激酶活性、检测细胞因子信息和分析hwin感染者CD8+ T细胞功能的关键。该中心还为用户开发了新的检测方法，包括改进的hiv特异性细胞毒性T淋巴细胞检测和组织细胞因子定量。该中心提供一系列服务，包括流式细胞术、淋巴细胞功能测定和共聚焦显微镜。关于使用仪器和数据解释的培训也是核心的一个关键组成部分。核心将由一个委员会监督。核心总监将负责核心的日常运行，并以标准化的方式规定使用的优先次序，其中包括适当的退款系统。预计贝勒医学院和德克萨斯大学、utmb加尔维斯顿分校、德克萨斯农工大学和杜兰大学医学院的至少19名研究人员将受益。～

The CFAR 1rnmunology Core is designed to provide both state-of-the-art flow cytometric capabilities as well as expertise and technical assistance for numerous immunological assays desired by members of the CFAR. The core is dedicated to providing such service in a timely, cost-effective, and efficient manner. The core is headed by Dr. Dorothy E. Lewis, who has over 20 years of flow and immunologic experience and a staff of well- trained personnel. The core consists of two flow cytometers, an analytical one and a high speed cell sorter, a BL-2 containment room for immunological assays, and a confocal microscope. Cytokine measurement expertise is provided by Dr. Reuben at M.D. Anderson Cancer Center, located across the street from Baylor College of Medicine, on a fee-for- service basis. In the previous funding period, the core has been essential for studies involving immune function in pediatric HW and has facilitated interactions with clinical and basic science researchers. In particular, the core was key for studies of Tat kinase activity in primary lymphocytes, in detection of cytokine messages, and in analysis of CD8+ T cell function in HWinfected children. The core has also developed new assays for the users, including improved HIV-speciflc cytotoxic T lymphocyte assays and cytokine quantitation in tissues. A range of services is provided by this core which include flow cytometry, lymphocyte function assays and confocal microscopy. Training on the use of instrumentation and data interpretation is also a key component of the core. The core will be overseen by a committee. The Core Director will have responsibility for day-to-day running of the core, and priorities for usage are prescribed in a standardized manner mcluding an appropriate charge-back system. It is anticipated that at least 19 investigators at Baylor College of Medicine and at The University of Texas, UTMB-Galveston, Texas A&M University, and Tulane University School of Medicine will benefit. ~