CORE--IMMUNOLOGY FACILITY

核心--免疫学设施

• 批准号: 6665548
• 负责人: DOROTHY E. LEWIS
• 金额: $ 12.95万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665548
• 关键词: AIDS; biomedical facility; clinical research; confocal scanning microscopy; cytokine; cytotoxic T lymphocyte; flow cytometry; human immunodeficiency virus; human tissue; immunologic assay /test; immunology; training

The CFAR 1rnmunology Core is designed to provide both state-of-the-art flow cytometric capabilities as well as expertise and technical assistance for numerous immunological assays desired by members of the CFAR. The core is dedicated to providing such service in a timely, cost-effective, and efficient manner. The core is headed by Dr. Dorothy E. Lewis, who has over 20 years of flow and immunologic experience and a staff of well- trained personnel. The core consists of two flow cytometers, an analytical one and a high speed cell sorter, a BL-2 containment room for immunological assays, and a confocal microscope. Cytokine measurement expertise is provided by Dr. Reuben at M.D. Anderson Cancer Center, located across the street from Baylor College of Medicine, on a fee-for- service basis. In the previous funding period, the core has been essential for studies involving immune function in pediatric HW and has facilitated interactions with clinical and basic science researchers. In particular, the core was key for studies of Tat kinase activity in primary lymphocytes, in detection of cytokine messages, and in analysis of CD8+ T cell function in HWinfected children. The core has also developed new assays for the users, including improved HIV-speciflc cytotoxic T lymphocyte assays and cytokine quantitation in tissues. A range of services is provided by this core which include flow cytometry, lymphocyte function assays and confocal microscopy. Training on the use of instrumentation and data interpretation is also a key component of the core. The core will be overseen by a committee. The Core Director will have responsibility for day-to-day running of the core, and priorities for usage are prescribed in a standardized manner mcluding an appropriate charge-back system. It is anticipated that at least 19 investigators at Baylor College of Medicine and at The University of Texas, UTMB-Galveston, Texas A&M University, and Tulane University School of Medicine will benefit. ~

CFAR 1免疫学核心旨在为CFAR成员所需的众多免疫学检测提供最先进的流式细胞仪功能以及专业知识和技术援助。该核心致力于以及时、具有成本效益和高效的方式提供此类服务。核心由多萝西·E博士领导。刘易斯，谁拥有超过20年的流动和免疫学的经验和一个训练有素的人员的工作人员。核心包括两台流式细胞仪，一台分析型流式细胞仪和一台高速细胞分选仪，一个用于免疫测定的BL-2密闭室和一台共聚焦显微镜。细胞因子测量专业知识由医学博士Reuben博士提供安德森癌症中心，位于贝勒医学院对面，按服务收费。在上一个资助期，该核心对于涉及儿科HW免疫功能的研究至关重要，并促进了与临床和基础科学研究人员的互动。特别是，核心是关键的研究达特激酶活性在初级淋巴细胞，在检测细胞因子的信息，并在分析的CD 8 + T细胞功能在HW感染的儿童。该中心还为用户开发了新的检测方法，包括改进的HIV特异性细胞毒性T淋巴细胞检测和组织中细胞因子定量。该中心提供一系列服务，包括流式细胞术、淋巴细胞功能测定和共聚焦显微镜。关于仪器使用和数据解释的培训也是核心的一个关键组成部分。核心将由一个委员会监督。 核心主任将负责核心的日常运作，并以标准化方式规定使用的优先次序，包括适当的收费制度。预计贝勒医学院和德克萨斯大学、UTMB-Galveston、德克萨斯A&M大学和杜兰大学医学院的至少19名研究人员将受益。~