CORE--IMMUNOLOGY FACILITY

本提案描述了纽约大学CFAR免疫学核心将提供的设施和服务。这一核心的主要目的是为艾滋病研究界的研究人员提供细胞免疫功能分析、艾滋病毒中和活性的测量、针对艾滋病毒结构蛋白的人类单抗的供应、啮齿动物单抗的生产，以及关于分析性能和人类单抗试剂生产的培训。这一核心的服务可供纽约大学艾滋病研究社区、纽约大学其他领域的研究人员(如纽约大学癌症中心成员)、纽约大学以外的NIH支持的研究人员以及世界各地需要mAbs进行有价值的艾滋病毒相关项目的科学家使用。在核心进行的分析包括(但不限于)对几种抗原或有丝分裂原之一的淋巴增殖、外周血单核细胞(PBMC)体外产生抗体、CD8细胞抑制病毒生长以及细胞毒性T淋巴细胞分析。其他服务包括使用几种分析方法测量血清中抗体介导的艾滋病毒中和活性和制备单抗。此外，我们还培训了来自纽约大学和其他美国及国际研究机构和大学的许多人进行这些不同的检测。Core还向世界各地的研究人员提供了70多种抗HIV人类单抗。单抗是不收费的(因为在大学和国外开账单很复杂)，但已经建立了一个用于免疫学和中和分析的收费系统。关于未来的建议是基于过去10年CFAR供资方面发展的专门知识和不断变化的需求，以及当地、国家和国际调查人员的需求。

This proposal describes the facilities and services to be provided by the Immunology Core of the NYU CFAR. The primary purpose of this Core is to provide investigators within the AIDS research community access to assays of cellular immune function measurements of HIV neutralizing activity, supplies of human monoclonal antibodies (mAbs) specific for structural proteins of HIV, production of rodent mAbs, and training on the performance of assays and production of human monoclonal reagents. The services of this core are available to the NYU AIDS research community, to NYU researchers in other fields (such as members of the NYU Cancer Center), to NIH-supported researchers outside of NYU, and to those scientists around the world who need mAbs for meritorious HIV- related projects. The assays performed in the Core include (but are not limited to) lymphoproliferation to one of several antigens or mitogens, in vitro antibody production by peripheral blood mononuclear cells (PBMCs), inhibition of virus growth by CD8 cells, and cytotoxic T lymphocyte assays. Additional services include measurement of Ab- mediated HIV neutralizing activity in sera and preparations of mAbs using several assays. In addition, we have trained many individuals, from NYU and other American and international research institutes and universities in the conduct of these various assays. The Core also provides more than 70 anti-HIV human mAbs to researchers around the world. No charge is made for the mAbs (because of the complexity of billing outside of the university and outside of the country), but a charge-back system has been established for immunologic and neutralization assays. The proposal for the future is based on the expertise developed and the evolving needs over the past 10 years of CFAR funding and upon the needs of local, national and international investigators.