Recovery of HIV Monoclonal Antibody Inventory Lost after Hurricane Sandy

描述（由申请人提供）：自1989年Zolla-Pazner博士发表第一篇描述HIV-1人单克隆抗体（mAb）的论文以来，她已将这些试剂提供给HIV研究界。迄今为止，已经制备了超过100种单克隆抗体，并进行了免疫化学和功能表征。这些包括来源于被不同进化枝感染的个体的细胞的人mAb（A、B、C、D、CRF01_AE、CRF02_AG等）。对p24、gp 41（簇1和2）和gp 120（V2、V3、C2、C5、CD 4 bs和V2 V3四元中和表位）中的表位具有特异性。此外，已经产生了对CMV、MuLV gp 70、炭疽保护性抗原（PA）、霍乱毒素B和细小病毒B19具有特异性的mAb，其经常用作抗HIV mAb的阴性对照。多年来，向艾滋病研究界提供这些mAb（约200 mg/年）得到了纽约大学艾滋病研究中心的支持。自2008年以来，通过Fisher BioServices（FBS-0022 C-05）的一项合同提供了提供这些试剂的部分资金，该合同直接与NIH签订合同，以维持NIH艾滋病研究和试剂储存库计划（ARRRP）。我们的实验室中冷冻了100多条mAb生产线。从这些细胞系中产生上清液并储存，直至储存库或个别科学家要求。上清液用作纯化所需IgG mAb的源材料，所述IgG mAb在运输前被定量并进行质量控制检测。当超级风暴桑迪（12年10月29日）导致断电时，我们实验室储存了来自38个mAb生产细胞系的62 L上清液。必须恢复这一库存，以便继续向艾滋病毒研究界提供这些试剂。该应用程序请求支持以补充该库存，这需要购买许多细胞系生长以及纯化、定量所需的大量供应品。 和mAb的质量保证测试，沿着在PI实验室工作超过20年的全职、高素质和经验丰富的高级研究技术员的工资。

DESCRIPTION (provided by applicant): Since the publication of her first paper describing human monoclonal antibodies (mAbs) to HIV-1 in 1989, Dr. Zolla-Pazner has made these reagents available to the HIV research community. To date, more than 100 mAbs have been made and immunochemically and functionally characterized. These include human mAbs derived from the cells of individuals infected with diverse clades, (A, B, C, D, CRF01_AE, CRF02_AG, etc.) with specificities for epitopes in p24, gp41 (clusters 1 and 2), and gp120 (V2, V3, C2, C5, CD4bs, and the V2V3 quaternary neutralizing epitope). In addition, mAbs have been generated that are specific for CMV, MuLV gp70, protective antigen (PA) of anthrax, cholera toxin B, and parvovirus Bl 9 which are frequently used as negative controls for anti-HIV mAbs. For many years, the provision of these mAbs (~200 mg/year) to the AIDS research community was supported by the NYU Center for AIDS Research. Since 2008, partial funding for the provision of these reagents has been provided via a subcontract from Fisher BioServices (FBS-0022C-05) which contracts directly with the NIH to maintain the NIH AIDS Research and Reagent Repository Program (ARRRP). Over 100 mAb-producing lines are frozen in our lab. Supernatants are produced from these lines and stored until requested by the Repository or individual scientists. The supernatants serve as the source material for purifying the needed IgG mAbs which are quantified and subjected to quality control testing prior to being shipped. Sixty-two liters of supernatants from 38 mAb-producing cell lines were in storage at our lab when electricity was lost as a result of Super Storm Sandy (10/29/12). This inventory must be restored in order to continue the provision of these reagents to the HIV research community. This application requests support to replenish this inventory which requires the purchase of the extensive supplies needed for the growth of the many cell lines, and the purification, quantitation and quality assurance testing of the mAbs, along with the salary of a full time, highly qualified and experienced Senior Research Technician who has worked in the PI'S lab for >20 years.