VIRAL IMMUNOLOGY CORE

病毒免疫核心

• 批准号: 6936130
• 负责人: Susan B Zolla-Pazner
• 金额: $ 54.49万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936130
• 关键词: AIDS; HIV infections; biomedical facility; cellular immunity; enzyme linked immunosorbent assay; human immunodeficiency virus; human subject; humoral immunity; immune response; immunity; immunogenetics; immunologic assay /test; radiotracer; virus infection mechanism; virus load

CORE E DESCRIPTION (provided by applicant): This proposal describes the facilities and services to be provided by the Viral Immunology Core of the NYU CFAR. The primary purpose of this Core is a) Provision of a range of assays to measure the function and characteristics of various cells involved in immune defense against HIV, the function of cells affected by HIV infection, and, the HLA type of cells (when this information is needed to perform various cellular assays); b) Testing of polyclonal sera as well as human, primate and rodent anti-HIV monoclonal antibodies (mAbs) for anti-HIV activity, for neutralizing activity against a wide range of HIV primary isolates, and for their ability to bind to intact virions and to infected cells; c) Virus isolation and characterization so that these viruses can be used in the aforementioned immunologic assays; d) Construction and production of pseudoviruses for use in the aforementioned assays; e) Provision of human anti-HIV mAbs to CFAR investigators and AIDS researchers around the world; f) Sequencing of Ig and T cell receptor genes for studies of gene usage in immune responses in the setting of HIV infection; and g) Provision of training to investigators, students, postdocs, international fellows, Infectious Disease fellows, and technicians, within and outside of CFAR, in the techniques enumerated above. The services of this core are available to the NYU AIDS research community, to NYU researchers in other fields (such as members of the NYU Cancer Center), to NIH supported researchers outside of NYU, and to those scientists around the world who need mAbs for meritorious HIV-related projects. A charge-back system is in place and contributes to the support of the services provided.

核心描述（由申请人提供）：该提案描述了纽约大学 CFAR 病毒免疫学核心将提供的设施和服务。该核心的主要目的是 a) 提供一系列测定，以测量参与 HIV 免疫防御的各种细胞的功能和特征、受 HIV 感染影响的细胞的功能以及细胞的 HLA 类型（当需要此信息来执行各种细胞测定时）； b) 测试多克隆血清以及人类、灵长类动物和啮齿类动物的抗 HIV 单克隆抗体（mAb）的抗 HIV 活性、针对各种 HIV 原代分离株的中和活性以及它们与完整病毒体和受感染细胞结合的能力； c) 病毒分离和表征，以便这些病毒可用于上述免疫学测定； d) 用于上述测定的假病毒的构建和生产； e) 向 CFAR 调查人员和 AIDS 提供人类抗 HIV 单克隆抗体 世界各地的研究人员； f) Ig 和 T 细胞受体基因测序，用于研究 HIV 感染情况下免疫反应中基因的使用； g) 向 CFAR 内外的研究人员、学生、博士后、国际研究员、传染病研究员和技术人员提供上述技术方面的培训。该核心的服务面向纽约大学艾滋病研究界、纽约大学其他领域的研究人员（例如纽约大学癌症中心的成员）、美国国立卫生研究院支持的纽约大学以外的研究人员，以及世界各地需要单克隆抗体来开展艾滋病毒相关项目的科学家。退款系统已到位，有助于支持所提供的服务。