权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

VIBRIO GENE EXPRESSION DURING INFECTION

感染期间的弧菌基因表达

基本信息

批准号：
6373551
负责人：
Andrew Camilli
金额：
$ 11.95万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-05-01 至 2003-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6373551
关键词：
Vibrio cholerae bacterial cytopathogenic effect bacterial genetics bacterial proteins electroporation gastrointestinal infection gene deletion mutation gene expression host organism interaction infant animal laboratory mouse molecular cloning northern blottings operon vibriosis virulence

项目摘要

DESCRIPTION (Adapted from the applicant's abstract): The pathogenicity of Vibrio cholerae, which infects mucosal surfaces in the small intestine, is believed to be a coordinated and multifactorial process. Unfortunately, knowledge of which genes are expressed during infection, as well as the regulatory proteins and host signals that govern their regulation, is rudimentary. A novel gene reporter system has been constructed for identifying in vivo induced genes of V. cholerae, and has been used to identify the vie (V. cholerae in vivo expressed) operon. vie is predicted to encode three two-component signal transduction proteins, including a sensor and two response regulators, the first such system described in V. cholerae. vie is unique among such systems both in its operon structure and its exclusive expression during infection. Based on analogy to other systems, it is hypothesized that the Vie proteins function by sensing environmental conditions in the host intestine and responding by regulating other genes. Characterization of vie thus provides a unique opportunity both to probe the host signals and bacterial regulatory proteins that control V. cholerae growth and pathogenicity as well as to allow investigation of vie-regulated genes. The goals of this research proposal are to characterize the vie operon and to identify and characterize vie-regulated genes. Mutations will be constructed in both vie and vie-regulated genes, and the effects of each on pathogenicity will be measured. The subcellular locations of the Vie proteins will be determined using immunochemical methods. The spatial and temporal expression patterns of the vie operon and vie-regulated genes will be determined during infection. This work may contribute to development of mucosal vaccines and to identification of new targets for therapies against mucosal pathogens.

描述（改编自申请人的摘要）：致病性霍乱弧菌感染小肠粘膜表面，被认为是一个协调的、多因素的过程。很遗憾，了解哪些基因在感染过程中表达，以及调节蛋白和控制其调节的宿主信号是简陋的。构建了一种新型基因报告系统鉴定霍乱弧菌体内诱导基因，并已用于识别 vie（霍乱弧菌体内表达）操纵子。 vie被预测编码三个双组分信号转导蛋白，包括传感器和两个响应调节器，V 中描述的第一个此类系统。霍乱。 vie 在此类系统中的独特之处在于其操纵子结构和其在感染期间的独特表达。基于与其他的类比系统中，假设 Vie 蛋白通过感知来发挥作用宿主肠道的环境条件并通过调节做出反应其他基因。因此，vie 的表征提供了一个独特的机会两者都可以探测宿主信号和细菌调节蛋白控制霍乱弧菌的生长和致病性，并允许 vi调控基因的研究。本研究计划的目标是表征 vie 操纵子并识别和表征 vie调控的基因。突变将在 vie 和 vie 调节的基因，每个基因对致病性的影响将是测量。 Vie 蛋白的亚细胞位置将被确定使用免疫化学方法。空间和时间表达模式 vie操纵子和vie调控基因的确定将在感染。这项工作可能有助于粘膜疫苗和疫苗的开发确定针对粘膜病原体的治疗新靶点。