CHARACTERIZATION OF THE GTPASE, SRBETA

GTPASE、SRBETA 的表征

• 批准号: 6518862
• 负责人: Joseph Stephen Glavy
• 金额: $ 2.31万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6518862
• 关键词: Escherichia coli; SDS polyacrylamide gel electrophoresis; X ray crystallography; density gradient ultracentrifugation; endoplasmic reticulum; fungal proteins; gel filtration chromatography; guanosinetriphosphatases; nuclear membrane; protein signal sequence; protein structure function; protein transport; ribonucleoproteins; western blottings

Cotranslational targeting and insertion of proteins into the protein conducting channel (PCC) of the endoplasmic reticulum (ER) requires the action of three interacting GTPases; SRP54, and the SRP receptor heterodimer SRalpha, and SRbeta. The SRbeta is one component of this pathway for which little is known. The proposed research will use a combination of biochemical analysis and X-ray crystallography to characterize the function and the structure of SRbeta alone and as a unit of the SRP receptor. The identification of regions involved in the interactions and transmission of signals will serve as a major tool in elucidating the mechanisms by which the combination of GTPases regulates protein translocation.

蛋白质共翻译靶向和插入内质网 (ER) 的蛋白质传导通道 (PCC) 需要三种相互作用的 GTP 酶的作用； SRP54 和 SRP 受体异二聚体 SRalpha 和 SRbeta。 SRbeta 是该途径的一个组成部分，但对其知之甚少。 拟议的研究将结合生化分析和 X 射线晶体学来表征 SRbeta 的功能和结构以及作为 SRP 受体单元的功能和结构。 识别参与信号相互作用和传递的区域将成为阐明 GTP 酶组合调节蛋白质易位机制的主要工具。