HALF-LIVES OF NUCLEAR PORE COMPLEX PROTEINS IN HUMAN CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了了解核孔复合体的组装和维护，有必要确定其组件的周转率。利用重同位素代谢标记、在细胞培养中增加时间(0、3、6、10、15、22和32小时)后免疫分离Nup107蛋白复合体，以及质谱分析，我们跟踪了Nup107及其相关蛋白的半衰期。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to gain understanding on the assembly and maintenance of the nuclear pore complex it is of interest to determine the turnover of its components. Using heavy-isotope metabolic labeling, immunoisolation of the Nup107 protein complex after increasing lengths of time (0, 3, 6, 10, 15, 22, and 32 hours)in cell culture, and mass spectrometry, we followed the half-lives of Nup107 and associated proteins.