Regulation of Pore Membrane Proteins during NPC Release and Dispersal in Open Mitosis

开放有丝分裂中 NPC 释放和分散过程中孔膜蛋白的调控

• 批准号: 9492347
• 负责人: Joseph Stephen Glavy
• 金额: $ 30.21万
• 依托单位: UNIVERSITY OF TEXAS TYLER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9492347
• 关键词: Regulation; Pore; Membrane; Proteins; during

Abstract The functional integrity of the nuclear pore complex (NPC) is achieved through the fastening to the pore membrane (POM) proteins. This interlocked zipper of proteins is important to maintain nuclear transport and allow proper progression through the cell cycle. NPCs are disassembled into nucleoporins (Nup) subcomplexes during entry into open mitosis in higher eukaryotic cells. POM proteins have been shown to affect cell cycle progression and to be involved in several disease states such as leukemia, heart disease and types of progeria. We hypothesize that Nup/POM interfaces are regulated by mitotic kinases at disassembly leading to their release and mitotic localization. To study this we propose: The determination of key phosphorylation interface targets and pairing them as substrates for kinases involved in disassembly and distribution of the NPC. We will identify and test these regulated areas including constraint-based modeling from our phosphorylation/interaction mapping. This work will be the key prerequisite to understand NPC regulation and distribution throughout the cell cycle.

摘要

项目成果

Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope.

从核膜中纯化 Cdk-CyclinB-激酶靶向磷酸肽。

• DOI: 10.1007/978-1-0716-2337-4_18
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Blethrow,JustinD;DiGuilio,AmandaL;Glavy,JosephS
• 通讯作者: Glavy,JosephS

Novel spiral structured nerve guidance conduits with multichannels and inner longitudinally aligned nanofibers for peripheral nerve regeneration.

• DOI: 10.1002/jbm.b.34233
• 发表时间: 2019-07
• 期刊: Journal of biomedical materials research. Part B, Applied biomaterials
• 作者: Shah MB;Chang W;Zhou G;Glavy JS;Cattabiani TM;Yu X
• 通讯作者: Yu X

Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy

• DOI: 10.1093/nar/gkz666
• 发表时间: 2019-09-19
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Dimas, Rey P.;Jordan, Benjamin R.;Chan, Clement T. Y.
• 通讯作者: Chan, Clement T. Y.