Optical Trapping

光捕获

• 批准号: 6485851
• 负责人: ROBERT JOSEPHS
• 金额: $ 20万
• 依托单位: ARRYX, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-17 至 2004-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485851
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; charge coupled device camera; computer human interaction; computer program /software; evaluation /testing; holography; lasers; light microscopy; liquid crystal; mathematics; nanotechnology; optics; sample collection; video recording system

Optical trapping with laser tweezers has proven to be a useful device for manipulating objects on a microscopic scale. Many systems of interest require multiple optical traps and several methods have been developed to achieve multiple trap configurations. However all of these methods for increasing the number of traps make the optical system increasingly more complex. Consequently currently available trapping systems can produce at most only a few independent traps. Recently, Grier and Dufresne conceived of a new solution for achieving a multi- trap system. In their method a hologram is used to alter a single laser beam's wavefront. The wavefront is altered so that the downstream laser beam forms a large number of individual laser beams with relative positions and directions of travel fixed by the exact nature of the hologram. The hologram can be calculated from a user specified pattern of desired trap positions. Currently a laboratory prototype has been fabricated. This prototype has demonstrated the ability create the holograms and to trap multiple (up to 200) particles in any chosen p Item. The prototype has not been optimized for use by individuals without expertise in optics. We propose developing a commercial version of the holographic optical tweezers (HOT) along with a sample chamber suitable for introducing, manipulating and collecting specimens. PROPOSED COMMERCIAL APPLICATION: Not available

事实证明，用激光镊子进行光学诱捕是一种有用的设备，用于在微观刻度上操纵对象。许多感兴趣的系统都需要多个光学陷阱，并且已经开发了几种方法来实现多个陷阱配置。但是，所有这些用于增加陷阱数量的方法使光学系统越来越复杂。因此，当前可用的陷阱系统最多只能产生几个独立的陷阱。最近，Grier和Dufresne构想了一种新的解决方案，以实现多陷阱系统。在他们的方法中，全息图用于改变单个激光束的波前。波前会改变，以使下游激光束形成大量的单个激光束，其相对位置和通过全息图的精确性固定的行进方向。全息图可以根据所需的陷阱位置的用户指定模式计算。目前已经制造了实验室原型。该原型已经证明了创建全息图的能力，并在任何选定的P项目中捕获多个（最多200）粒子。该原型尚未被优化，用于没有光学专业知识的个人使用。我们建议开发全息光学镊子（HOT）的商业版，以及适合引入，操纵和收集样品的样品室。拟议的商业应用程序：不可用