AN ELECTRON MICROSCOPE FOR A MULTIUSER FACILITY

适用于多用户设施的电子显微镜

• 批准号: 3520064
• 负责人: ROBERT JOSEPHS
• 金额: $ 23.9万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-11 至 1989-01-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520064
• 关键词: biomedical equipment purchase; electron microscopy; image processing; molecular biology

A group of investigators working on biological structures seeks support to purchase an electron microscope for a University multiuser facility. The electron microscope will be used for studies of the structures of sickle hemoglobin polymers, the acetycholine receptor, the red blood cell cytoskeleton, the eryth- ropoietin receptor, cardiac gap junctions, and nucleoprotein complexes. These studies require a very high resolution microscope equipped with a goniometer stage and capable of cryo and low dose microscopy. The applicant group of investigators are now entirely dependent upon a single twenty-one year old Philips EM 300 for high resolution work. Even though this microscope is well maintained, age-related problems are resulting in ever increasing difficulties. The proposed new microscope would be installed in an existing EM lab which contains all the supporting equipment normally found in electron microscope laboratories. This lab is staffed by a very competent electron microscope technician. The laboratory has very extensive computer facilities for digital image processing which complement the EM hardware. All of this existing infrastructure and equipment would be available to the user group. The lab has maintained (and will continue to maintain) a policy of providing access to the EM facility for research and training for all who wish to use it.

一组从事生物结构的研究者寻求 支持为大学购买电子显微镜 多用户设施。 电子显微镜将用于 研究镰状血红蛋白聚合物的结构， 乙胆碱受体，红细胞细胞骨架，红色 ropoietin受体，心脏间隙连接和核蛋白蛋白 复合物。 这些研究需要很高的分辨率显微镜 配备了角度计阶段，能够冷冻和低剂量 显微镜。 申请者组现在完全取决于 在一个二十一岁的飞利浦EM 300上 决议工作。 即使该显微镜维护得很好， 与年龄有关的问题导致困难越来越大。 提出的新显微镜将安装在现有的EM中 实验室包含通常在 电子显微镜实验室。 这个实验室由一个非常 胜任的电子显微镜技术员。 实验室非常 数字图像处理的广泛计算机设施 补充EM硬件。 所有这些现有基础架构 用户组将使用设备。 实验室有 维护（并将继续维护）提供的政策 访问EM设施进行研究和培训的所有人 希望使用它。