HOPKINS DK CENTER FOR THE ANALYSIS OF GENE EXPRESSION

霍普金斯 DK 基因表达分析中心

• 批准号: 6381935
• 负责人: GREGORY G. GERMINO
• 金额: $ 57.23万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6381935
• 关键词: biomedical resource; biotechnology; cell differentiation; cell proliferation; clinical research; complementary DNA; diabetes mellitus genetics; epithelium; gene expression; human subject; inflammation; informatics; laboratory mouse; laboratory rat; microarray technology; statistics /biometry; stress

DESCRIPTION (Abstract of the application) Recent advances in genomic technologies have made possible the simultaneous examination of the expression patterns of a large number of genes in many biological systems. The principal approach, often called the "chip technology" involves the production of high-density arrays (microarrays) that contain thousands of previously isolated complementary DNA (cDNA) sequences and which are used to analyze gene expression on a global scale. The potential for gaining important and novel information on the fundamental mechanisms of biology and pathobiology with this technology is enormous. Our group is a consortium of investigators who are studying the biology-and pathobiology of the endocrine, renal or gastrointestinal system. To ensure that our research benefits from the power of rapidly advancing genomic techniques, we propose to form the Hopkins DK Center for the Analysis of Gene Expression. The Goals of this research application are (1) to perform genome-wide patterning of gene expression for the involved projects using cDNA microarrays, and (2) to establish a potentially important and powerful thematic approach to the analysis of gene expression among the concerned projects. The Specific Aim is to establish a core research facility at the Johns Hopkins University School of Medicine to support the production of cDNA microarrays and the analysis of gene profiles for investigators whose research is based on areas within the scientific mission of the NIDDK. The initial phase of the grant application will include 15 projects from established investigators and 7 pilot/feasibility projects from junior investigators. These projects share a programmatic emphasis on research that is focused on the molecular regulatory mechanisms in the biology and pathobiology of the renal, digestive, and endocrine organs. The projects can further be divided into 3 major themes that include: (1) The genomic responses to stress or inflammation, (2) Mechanisms regulating the proliferative and/or differentiated phenotype in epithelial cells, and (3) Cellular and molecular dysfunction in diabetes and other metabolic/endocrine disorders. The analysis of the array data will be supported by a Bioinformatics and a Biostatistics Core, staffed by local experts in the respective field. Thus, this application represents a rational and focused approach to the specific questions that each investigator addresses. The potential for gaining novel and relevant insights into the molecular mechanisms responsible for each of the three main themes of the proposed research through the use of the DK Center by the participating investigators is considerable.

说明（申请摘要） 基因组技术的最新进展使得同时 研究了许多基因的表达模式， 生物系统。主要的方法，通常被称为“芯片技术”， 涉及高密度阵列（微阵列）的生产， 数千个先前分离的互补DNA（cDNA）序列， 用于分析全球范围内的基因表达。的潜力 获得重要的和新的信息的基本机制， 在生物学和病理学上的应用是巨大的。我们组是一个 研究生物学和病理生物学的研究者联盟 内分泌、肾脏或胃肠道系统。以确保我们的研究 受益于快速发展的基因组技术的力量，我们建议 来自霍普金斯DK基因表达分析中心。的目标 该研究应用是（1）对基因进行全基因组模式化 使用cDNA微阵列表达所涉及的项目，和（2） 建立一个潜在的重要和强有力的专题方法， 分析相关项目的基因表达。具体目标是 在约翰霍普金斯大学医学院建立一个核心研究设施， 支持cDNA微阵列生产和基因分析的药物 其研究基于以下领域的研究者的简介： NIDDK的科学使命。赠款申请的初始阶段 将包括常设调查员的15个项目和7个试点/可行性项目 初级调查员的项目这些项目共享一个方案 强调研究的重点是分子调控机制， 肾脏、消化和内分泌器官的生物学和病理学。的 项目可进一步分为三大主题，包括：（1） 对应激或炎症的基因组反应，（2）调节 上皮细胞中的增殖和/或分化表型，和（3） 糖尿病和其他代谢/内分泌疾病中的细胞和分子功能障碍 紊乱阵列数据的分析将得到生物信息学 以及生物统计学核心，由相关领域的当地专家组成。 因此，该应用程序代表了一种理性和集中的方法， 每个调查员都要回答的具体问题。获得的潜力 新的和相关的见解的分子机制负责每个 通过使用DK，建议研究的三个主要主题 中心参与的研究者是相当多的。