VACCINES ELICITING CD8 CYTOTOXIC T CELL RESPONSES

引发 CD8 细胞毒性 T 细胞反应的疫苗

• 批准号: 6511093
• 负责人: HERMAN N EISEN
• 金额: $ 31.64万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6511093
• 关键词: AIDS vaccines; antigen presentation; chimeric proteins; cytotoxic T lymphocyte; flow cytometry; genetically modified animals; immunologic memory; laboratory mouse; simian immunodeficiency virus; stress proteins; vaccine development; vector vaccine; virus protein

Individuals infected with HIV-1 often make vigorous anti-viral CD8 cytotoxic T lymphocyte (CTL) responses. The CTL appear to play an important role in reducing the viral load but infection usually persists and eventually worsens. The long-term goal of this project to is facilitate the development of vaccines aimed at generating more effective CD8 CTL responses than occur in the course of natural infection. To this end we will define the maximally effective forms of heat shock fusion protein and DNA vectors as immunogens for eliciting potent primary and memory CD8 cytotoxic T lymphocyte (CTL) responses in a transgenic mouse model with aim of eventually applying related immunogens to the vaccination of nonhuman primates (macaque monkeys?) against SIV infection.

感染HIV-1的个体通常会产生强有力的抗病毒CD 8 细胞毒性T淋巴细胞（CTL）应答。 CTL似乎在扮演一个 在减少病毒载量方面起重要作用，但感染通常持续存在 最后变成了一堆垃圾。 该项目的长期目标是 促进疫苗的开发， 有效的CD 8 CTL应答比在自然免疫过程中发生的更有效。 感染 为此，我们将确定最有效的形式， 作为免疫原的热休克融合蛋白和DNA载体 有效的原发性和记忆性CD 8细胞毒性T淋巴细胞（CTL）应答， 一种转基因小鼠模型，目的是最终应用相关的 非人灵长类动物（猕猴？） 防止SIV感染。

项目成果

In vivo cytotoxic T lymphocyte elicitation by mycobacterial heat shock protein 70 fusion proteins maps to a discrete domain and is CD4(+) T cell independent.

分枝杆菌热休克蛋白70融合蛋白映射到离散结构域的体内细胞毒性T淋巴细胞诱导，并且与CD4（+）T细胞无关。

• DOI: 10.1084/jem.191.2.403
• 发表时间: 2000-01-17
• 期刊: JOURNAL OF EXPERIMENTAL MEDICINE
• 影响因子: 15.3
• 作者: Huang, Q;Richmond, J F;Suzue, K;Eisen, H N;Young, R A
• 通讯作者: Young, R A