ADVANCED MULTISPECTRAL IMAGING FOR MEDICAL DIAGNOSTICS

描述(逐字摘自申请者摘要)：这个项目将开发一个 一种新型的同步发光多光谱成像系统 (SL)在体内快速检测癌症的概念。该提案将解决 恶性肿瘤的在体实时识别与表征问题 以及上胃肠道的癌前组织。当存在的时候 目前，通过内窥镜很容易检测到巴雷特粘膜的病变 广泛的活检可发现不典型增生和早期癌症。典型的协议 每隔2厘米对巴雷特粘膜进行四个象限的活检。虽然这件事 是标准技术，它只提供3-5%的粘膜样本 可发现不典型增生和弥漫性癌的表面。其余97-95年度 粘膜的百分比没有被取样。激光诱导荧光(LIF) 光谱学已经被用来检测癌症和高度不典型增生 巴雷特食道。然而，该系统使用接触技术，该接触技术 每次测量时取样1毫米面积的组织。而接触式LIF系统 比夹点活检技术更好，需要一种新的系统来允许 对整个粘膜表面的检查。为了满足这一重要需求 在成像方面，将开发一套实时同步成像系统，基于 最先进的声光可调谐滤光器技术 内窥镜检查。将开发新的MSI成像技术以获得空间 已解决的图像的SL的性质略有不同的恶性肿瘤和 非恶性肿瘤。同步发光分析将大大简化 从组织中产生的荧光。这反过来将提供更快的 更准确的活体分析，而不需要活组织检查。其独特的成像方面 该MSI系统将提供实时空间信息，允许 对感兴趣的大范围区域进行全面诊断。随着……的发展 这项技术的初步研究将在两个模型系统上进行， 橡树岭国家实验室的活组织和实验动物 和田纳西大学。一旦系统被优化， 临床活体研究将在汤普森大学进行。 田纳西州诺克斯维尔的癌症生存中心(TCSC)。跨学科的 将使用一种方法来执行拟议的研究，以在 高效率和高成本效益的方式。项目人员包括 跨学科研究人员小组，在ORNL独一无二地提供，具有 在广泛的技术领域有经验：分子光谱学和 先进仪器、分子生物学、细胞生物学。 微电学、分子遗传学和小鼠研究。此外, 项目人员包括田纳西大学的一名兽医，以及 在TCSC拥有临床研究专业知识的医学研究人员。

DESCRIPTION (Verbatim from Applicant's Abstract): This project will develop a novel multi-spectral imaging (MSI) system using the synchronous luminescence (SL) concept to rapidly detect cancer in vivo. The proposal will address the problem of real-time in vivo identification and characterization of malignant and pre-malignant tissues in the upper gastrointestinal tract. While presence of Barrett's mucosa is simple to detect endoscopically, at the present time dysplasia and early cancer is found by extensive biopsies. The typical protocol is four quadrant biopsies at 2-cm intervals of the Barrett's mucosa. While this is the standard technique, it only provides 3-5 percent sampling of the mucosal surface where dysplasia and diffuse cancer may be found. The remaining 97-95 percent of the mucosa is not sampled. Laser-induced fluorescence (LIF) spectroscopy has already been used to detect cancer and high grade dysplasia in Barrett's esophagus. However, that system uses a contact technique which samples a 1 mm area of tissue at each measurement. While the contact LIF system is better than the pinch biopsy technique, a new system is needed to allow examination of the entire surface of the mucosa. To address this important need in imaging, a real-time synchronous imaging system will be developed, based on state-of-the-art acousto-optic tunable filter technology coupled to an endoscope. Novel MSI imaging technology will be developed to obtain spatially resolved images of the slight differences in SL properties of malignant versus non-malignant tumors. Synchronous luminescence analysis will greatly simplify the resulting fluorescence from the tissue. This in turn will provide a faster and more accurate in vivo analysis without biopsy. The unique imaging aspect of this MSI system will provide real-time spatial information, allowing for comprehensive diagnosis of large areas of interest. Following development of this technology, initial studies will be performed on two model systems, biopsied tissues as well as laboratory animals at Oak Ridge National Laboratory (ORNL) and the University of Tennessee. Once the system has been optimized, clinical in vivo studies will be performed on human subjects at the Thompson Cancer Survival Center (TCSC) in Knoxville, Tennessee. An interdisciplinary approach will be used to perform the proposed research to provide results in an efficient and cost effective manner. Project personnel include an interdisciplinary group of researchers, uniquely available at ORNL, with experience in a wide range of technical areas: molecular spectroscopy and advanced instrumentation, molecular biology, cellular biology. microelectroptics and molecular genetics and mouse studies. In addition, project personnel include a veterinarian at the University of Tennessee, and medical researchers with expertise in clinical studies at the TCSC.