Nonobiosensors for Probing Chemical Exposure and Metabolism Pathways of Individua

用于探测个体化学暴露和代谢途径的非生物传感器

基本信息

批准号：
7628387
负责人：
Tuan Vo-Dinh
金额：
$ 32.37万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-17 至 2012-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Although the toxic nature of many chemicals, such as carcinogenic species, which have found their way into the environment is well-known, the mechanisms by which these chemicals exert their toxic effects at the cellular and molecular level are only now beginning to be understood. There is a strong need for developing a molecular tool that is capable of monitoring biochemical processes or detecting specific biomarkers in a living cell following chemical exposure. Minimally invasive analysis of cellular signaling pathways inside single intact cells is becoming increasingly important fundamentally because cells in a population respond asynchronously to external stimuli. The objective of this research is to conduct fundamental research and develop nanobiosensors for probing chemical exposure and health effects of individual living cells. The proposed technique could provide unprecedented insights into intact cell function, allowing studies of molecular functions in the context of the functional cell architecture. This proposed research will contribute to future advancements in biology and medicine, which is directly relevant to the mission of National Institute of Environmental Health Sciences (NIEHS). The specific aims of the project are: 1) Develop a nanosensor for monitoring the carcinogenic compound, benzo[a]pyrene (BaP) and the related biomarker of exposure, benzopyrene tetrol (BPT), 2) Develop dual-target sensing technology for the nanobiosensor to achieve simultaneous detection of BaP and BPT using phase-resolved detection, and 3) Investigation of exposure and metabolism pathways in a single living cell exposed to BaP. The nanoprobes to be developed in this project will open new horizons to a host of applications in biotechnology, molecular biology and environmental health research, and the study of in situ intracellular signaling processes, and investigations of the chemical transport and toxicity.

描述（由申请人提供）：尽管许多化学物质（例如致癌物种）的毒性性质是众所周知的，但这些化学物质在细胞和分子水平上施加毒性作用的机制才开始被理解。强烈需要开发能够监测生化过程或在化学暴露后在活细胞中检测特定生物标志物的分子工具。单个完整细胞内细胞信号通路的微创分析在根本上变得越来越重要，因为人群中的细胞对外部刺激异步反应。这项研究的目的是进行基础研究，并开发纳米传感器，以探测单个活细胞的化学暴露和健康影响。所提出的技术可以为完整的细胞功能提供前所未有的见解，从而可以在功能细胞结构的背景下研究分子功能。这项拟议的研究将有助于生物学和医学的未来进步，这与国家环境健康科学研究所（NIEHS）的使命直接相关。该项目的具体目的是：1）开发一种纳米传感器，用于监测致癌化合物，苯并[a] pyrene（BAP）和相关的暴露生物标志物，苯二苯甲酸酯四酚（BPT），2），使用双目标传感技术，以实现BBAP和BPPT的同时检测，以实现双目标传感技术，并实现BBAP和BPPT的研究。暴露于BAP的单个活细胞中的代谢途径。该项目中要开发的纳米探针将为生物技术，分子生物学和环境健康研究以及对原位的细胞内信号传导过程的研究以及化学运输和毒性的研究开辟新的范围。