Core--Toxin probes and assays

核心——毒素探针与检测

• 批准号: 6485255
• 负责人: Daniel G Baden
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485255
• 关键词: animal tissue; bioassay; biomedical facility; chemical kinetics; environmental health; environmental toxicology; fish; high performance liquid chromatography; marine toxins; nuclear magnetic resonance spectroscopy; synaptosomes

The Toxin Probes and Assays Core will provide analytical support to each of the four projects. Support will be in the form of (1) toxin standards, (2) anti-toxin antibodies and synaptosomes as reagents for specific binding assays, (3) toxin derivatives that may encompass metabolites, conjugates, and photolysis products, (4) Fourier transform infrared and nuclear magnetic and proton and carbon) resonance spectrometry of standards and synthetic metabolites or proto-products, and (5) high performance liquid chromatographic analysis. For Research Project 1, the core anticipates support items (1), photolytic (3), and (5) to be the most important. For Research Project 2, support items (1), (2), (3) and (5) will be essential. For Research Project (3) toxin derivatives that may encompass metabolites, conjugates, and photolysis products, (4) Fourier transform infrared and nuclear magnetic (proton and carbon) resonance spectrometry of standards and synthetic metabolites or proto- products, and (5) high performance liquid chromatographic analysis. For Research Project 1, the core anticipates support items (1), photolytic (3), and (5) to be the most important. For Research Project 2, support items (1), (2) , (3) and (5) will be essential. For Research Project 2, 3, support items (1) and (2) will provide the most benefit. For Research Project 4, support items (1) and (2) will be the most important. The core will also provide antibodies and reagent toxins to Facilities Core 2, the Immunocytochemistry Core, so that immunoperoxidase assays an be carried out relative to phagocytic accumulation and biomarker assay development. This relative to phagocytic accumulation and biomarker assay development. This latter effort will require synthesis of antibody- HRP conjugates and measurement of specific enzymatic activity per binding unit. To carry out this Program efficiently, Dr. Baden will oversee the core s functions, determine the level of effort to be expended on each project, identify the standards assays, and derivatives that will be of most use, and work with the 2 research associates to provide the materials and assay logistics and a timely manner. The two research associates will receive samples, isolate quantitatively and identify the components, develop synthetic methods to produce sufficient quantities for controlled experiments, and provide advice to individual projects pertaining to amounts of material to collect, methods of preservation, and methods of transport.

毒素探针和测定核心将为这四个项目提供分析支持。支持将以(1)毒素标准，(2)抗毒素抗体和突触体作为特定结合试验的试剂，(3)毒素衍生物可能包含代谢物，缀合物和光解产物，(4)傅立叶变换红外和核磁（质子和碳）共振光谱法的标准和合成代谢物或原产物，以及(5)高效液相色谱分析。对于研究项目1，核心预期支持项目(1)、光解(3)和(5)是最重要的。对于研究项目2，支持项目(1)、(2)、(3)和(5)将是必不可少的。研究项目(3)可能包含代谢物、缀合物和光解产物的毒素衍生物，(4)标准物和合成代谢物或原产物的傅立叶变换红外和核磁（质子和碳）共振光谱分析，以及(5)高效液相色谱分析。对于研究项目1，核心预期支持项目(1)、光解(3)和(5)是最重要的。对于研究项目2，支持项目(1)、(2)、(3)和(5)将是必不可少的。对于研究项目2,3，支持项目(1)和(2)将提供最大的利益。对于研究项目4，支持项(1)和(2)将是最重要的。该核心还将为设施核心2（免疫细胞化学核心）提供抗体和试剂毒素，以便进行免疫过氧化物酶检测，以进行吞噬积累和生物标志物检测开发。这与吞噬细胞积累和生物标志物测定的发展有关。后一项工作将需要合成抗体- HRP偶联物和测量每个结合单元的特定酶活性。为了有效地开展该项目，Baden博士将监督核心职能，确定每个项目的努力程度，确定最常用的标准分析方法和衍生品，并与2名研究助理合作，及时提供材料和分析物流。这两名研究助理将接收样品，定量分离并鉴定成分，开发合成方法以生产足够数量的受控实验，并就收集材料的数量、保存方法和运输方法向个别项目提供建议。