Core--Toxin Probes and Assays

核心——毒素探针与检测

• 批准号: 7131157
• 负责人: Daniel G Baden
• 金额: $ 33.66万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-08 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7131157
• 关键词: Dinoflagellates; analytical chemistry; animal tissue; antitoxins; bioassay; biomedical facility; brevetoxin; chemical registry /resource; environmental health; environmental toxicology; enzyme linked immunosorbent assay; fish; high performance liquid chromatography; immunocytochemistry; microorganism culture; radiotracer; reagent /indicator; spectrometry; synaptosomes

The Function of the Toxin Probes and Assays Core is to provide analytical support to each of the four Research Projects (RP1-4) and to the other Facility Core (FC2). Support will be in the form of (1) toxin standards, (2) toxin antibodies, synaptosomes and pulmonary receptors to be used as reagents for specific binding assays, (3) new toxin-related reagents that may include derivative, natural, or synthetic brevetoxin antagonists, new brevetoxins, mixtures mimicking natural bloom samples, and radiolabeled products, (4) spectroscopy (FTIR, 1-D & 2-D NMR, LC-MS and HR-MS) as structural chemistry support for materials isolated from aerosols, water, and mammalian tissues, (5) HPLC separation assistance, both quantitation and methods development, (6) detection expertise and assays for enzyme-linked immunoassay (ELISA) in the laboratory and in the field as well as ELISA kits provided to RPs in support of their laboratory activities requiring range-finding or very rapid turnaround, (7) imtnunocytochemical analyses of tissue and cell samples, and (8) isolation and cultivation of Karenia species present in active red tides to determine the toxigenic origin of brevetoxins.

毒素探针和测定核心的功能是为四项研究中的每一项提供分析支持， 项目（RP 1 -4）和其他设施核心（FC 2）。支持的形式将是（1）毒素标准， (2)毒素抗体、突触体和肺受体用作特异性结合测定的试剂， (3)新的毒素相关试剂，其可包括衍生物、天然或合成的短毒素拮抗剂， 新的短毒素，模拟天然水华样品的混合物，和放射性标记的产物，（4）光谱学（FTIR， 1-D和2-D NMR，LC-MS和HR-MS）作为从气溶胶中分离的材料的结构化学支持， 水和哺乳动物组织，（5）HPLC分离辅助，包括定量和方法开发， (6)实验室和现场的酶联免疫测定（ELISA）检测专业知识和分析 以及向RP提供的ELISA试剂盒，以支持其需要范围确定的实验室活动， 快速周转，（7）组织和细胞样品的免疫细胞化学分析，以及（8）分离和培养 的Karenia物种存在于活跃的赤潮，以确定短尾藻毒素的致癌来源。