CORE--MOLECULAR BIOLOGY

描述：分子生物学设施核心的总体目标是 为中心调查人员提供访问尖端和最先进的 分子技术。这一目标已经并将通过 以下具体目标：1)提供常规和专门的分子 生物服务，目前包括DNA分离、聚合酶链式反应、亚克隆、DNA 测序、基因分型和突变检测。2)提供指导和 协助使用成像采集和分析仪器； 序列数据分析和序列数据库搜索，以及提供 监督访问高通量测序、DNA片段分析和 机器人学。3)为各种分子提供专业知识和咨询 生物学方法论。4)确定、开发和实施新技术，以 满足中心调查人员的需求。此协作室核心已由 在当前资助期内的中心成员数量。通过一个 作为中心拨款的补充，他们能够从手册升级 通过购买ABI 377将排序操作转移到自动化设施 定序器。他们还扩大了基于聚合酶链式反应的基因分型服务。新的 已经获得了用于序列分析的计算机工作站和几个新的 核心增加了成像仪器，包括柯达图像 440CF站、分子动力学荧光成像仪SI和分子动力学 风暴820磷光成像仪。最近，这个核心已经转移到扩展和 新装修的空间提供了一个成像实验室，一个潮湿的 实验室，以及一个高吞吐量/自动化实验室。未来方向 包括扩大自动化和基因分型服务，以及工作 与拟议的功能基因组学设施核心开发密切相关 基因表达服务和DNA测序。

Description: The overall goal of the Molecular Biology Facility Core is to provide Center investigators access to sophisticated and state of the art molecular techniques. This goal has and will be accomplished through the following specific aims: 1) Provide both routine and specialized molecular biology services, currently including DNA isolation, PCR, subcloning, DNA sequencing, genotyping, and mutation detection. 2) Provide instruction and assistance in the use of imaging acquisition and analysis instruments, sequence data analysis, and sequence database searching, as well as provide supervised access to high throughput sequencing, DNA fragment analysis and robotics. 3) Provide expertise and consultation for a variety of molecular biology methodologies. 4) Identify, develop and implement new technologies to meet the needs of Center investigators. This Facility Core has been used by a number of Center members during the current funding period. Through a supplement to the Center grant, they were able to upgrade from a manual sequencing operation to an automated facility with the purchase of an ABI 377 sequencer. They also have expanded their PCR-based genotyping services. New computer workstations have been obtained for sequence analysis and several new imaging instruments have been added to the core, including a Kodak Image Station 440CF, a Molecular Dynamics FluorImager SI, and a Molecular Dynamics Storm 820 Phosphoimager. Most recently, this core has moved into expanded and newly renovated space that provides for an imaging laboratory, a wet laboratory, and a high throughput/automation laboratory. Future directions include expanded automation and genotyping services, as well as working closely with the proposed Functional Genomics Facility Core in the development of gene expression services and DNA sequencing.