Facility Core B: Molecular Biology

总目标和具体目标 分子生物学设施核心的总体目标是为中心研究人员提供访问 先进和最先进的分子技术、服务和仪器。因为中心是 在多学科性质上，不同中心调查人员所需的支持程度差别很大。 因此，分子生物学设施核心提供的访问级别因咨询和 通过提供关于特定技术和/或工具的培训，就项目的具体方面提供咨询 核心，完成分子分析从样品准备到最终数据的分析和解释。 设施核心1的具体目标是： 1.继续提供分子生物学服务和检测，以回应中心的需求 成员，包括各种DMA水平的技术、比较表达分析和蛋白质 分离、鉴定和分析； 2.指导和协助中心调查实验室使用宏观 用于数字图像采集和分析、序列数据的成像设备和计算机软件 分析，并搜索序列数据库； 3.提供实时聚合酶链式反应、高通量测序、片段分析和 机器人设备； 4.为广泛的分子生物学程序提供专门知识、咨询和培训， 尤其是在基因表达和蛋白质表达技术方面； 5.识别、引进/开发和实施新技术和新检测方法以满足需求 中心成员的需求和新的中心计划的新需求。

Overall Objectives and Specific Aims The overall goal of the Molecular Biology Facility Core is to provide Center Investigators access to sophisticated and state-of-the-art molecular techniques, services, and instrumentation. Because the Center is multidisciplinary in nature, the level of support needed by different Center investigators varies widely. Consequently, the level of access offered by the Molecular Biology Facility Core varies from consultation and advice on specific aspects of projects, through training on particular techniques and/or instruments available in the Core, to complete molecular analyses from sample preparation to the final data analysis and interpretation. The Specific Aims of Facility Core 1 are: 1. to continue to provide molecular biology services and assays in response to the needs of Center Members, including a variety of DMA level techniques, comparative expression analysis and protein separation, identification and analysis; 2. to provide instruction and assistance to Center investigator laboratories in the use of macroscopic image equipment and computer software for digital image acquisition and analysis, sequence data analysis, and sequence database searching; 3. to provide supervised access to realtime PCR, high throughput sequencing, fragment analysis, and robotic equipment; 4. to provide expertise, consultation and training for a wide range of molecular biology procedures, especially in gene expression and protein expression techniques; 5. to identify, introduce/develop, and implement new technologies and new assays to meet the needs of Center Members and the emerging needs of new Center initiatives.