CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6442963
• 负责人: Jianjun Shen
• 金额: $ 11.79万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442963
• 关键词: DNA; biomedical facility; environmental health; environmental toxicology; gene mutation; genotype; high throughput technology; molecular biology; molecular cloning; nucleic acid sequence; polymerase chain reaction

Description: The overall goal of the Molecular Biology Facility Core is to provide Center investigators access to sophisticated and state of the art molecular techniques. This goal has and will be accomplished through the following specific aims: 1) Provide both routine and specialized molecular biology services, currently including DNA isolation, PCR, subcloning, DNA sequencing, genotyping, and mutation detection. 2) Provide instruction and assistance in the use of imaging acquisition and analysis instruments, sequence data analysis, and sequence database searching, as well as provide supervised access to high throughput sequencing, DNA fragment analysis and robotics. 3) Provide expertise and consultation for a variety of molecular biology methodologies. 4) Identify, develop and implement new technologies to meet the needs of Center investigators. This Facility Core has been used by a number of Center members during the current funding period. Through a supplement to the Center grant, they were able to upgrade from a manual sequencing operation to an automated facility with the purchase of an ABI 377 sequencer. They also have expanded their PCR-based genotyping services. New computer workstations have been obtained for sequence analysis and several new imaging instruments have been added to the core, including a Kodak Image Station 440CF, a Molecular Dynamics FluorImager SI, and a Molecular Dynamics Storm 820 Phosphoimager. Most recently, this core has moved into expanded and newly renovated space that provides for an imaging laboratory, a wet laboratory, and a high throughput/automation laboratory. Future directions include expanded automation and genotyping services, as well as working closely with the proposed Functional Genomics Facility Core in the development of gene expression services and DNA sequencing.

描述：分子生物学设施核心的总体目标是 为中心调查人员提供先进且最先进的信息 分子技术。这一目标已经并将通过以下方式实现 以下具体目标： 1) 提供常规和专门的分子 生物学服务，目前包括DNA分离、PCR、亚克隆、DNA 测序、基因分型和突变检测。 2) 提供指导并 协助使用成像采集和分析仪器， 序列数据分析，序列数据库检索，并提供 监督访问高通量测序、DNA 片段分析和 机器人技术。 3）提供各种分子的专业知识和咨询 生物学方法论。 4) 识别、开发和实施新技术 满足中心调查人员的需求。该设施核心已被使用 当前资助期间的中心成员数量。通过一个 作为中心补助金的补充，他们能够从手动升级 购买 ABI 377 即可对自动化设施进行测序操作 定序器。他们还扩展了基于 PCR 的基因分型服务。新的 已获得用于序列分析的计算机工作站和一些新的 成像仪器已添加到核心中，包括柯达图像 Station 440CF、分子动力学 FluorImager SI 和分子动力学 Storm 820 磷成像仪。最近，该核心已进入扩展和 新装修的空间提供了一个成像实验室、一个湿室 实验室和高通量/自动化实验室。未来方向 包括扩展的自动化和基因分型服务，以及工作 与开发中拟议的功能基因组学设施核心密切相关 基因表达服务和 DNA 测序。