Spider silk production using a controlled laboratory system

在我的项目中，我将尝试创造一种生产蜘蛛丝的系统，希望能提供一种更好、更可靠的工业化生产方法。我建议的系统将是从诺丁汉地区的圆球编织物种的腹部解剖出单个丝腺，并在培养过程中保留它们的腺体。这个体外系统将使我能够更准确地研究细胞丝素生产的机制。一旦实现了这一点，我将尝试用丝腺创造细胞系，并在实验室条件下使用天然蛋白质机器生产天然丝绸。这个系统将解决之前的尝试所面临的问题，因为它将生产出生理上相同的丝绸涂料，这些丝绸涂料将经过与天然丝绸完全相同的后处理、包装和排泄系统。此外，它将是100%的真丝涂料，消除了任何昂贵或耗时的净化过程。这将允许兴奋剂在生产后不久直接纺丝成纤维。通过使用丝腺细胞制造人造腺体，我们有望快速生产工业规模的本地蜘蛛丝。使用天然氨基酸前体，我们将能够生产一种天然的、碳中性的、强大的、灵活的、对环境影响小的生物聚合物。

In my project, I will attempt to create a system for producing spider silk that will hopefully provide a better and more reliable method of industrial production. The system I propose will be to dissect out the individual silk glands from the abdomens of orb-weaving species from the Nottingham area and maintain their glands in culture. This ex-vivo system will allow me to more accurately study the mechanisms of cellular silk production. Once this has been achieved I will attempt to create cell lines from the silk glands and use the native protein machinery to produce native silk under laboratory conditions. This system will address the problems faced by previous attempts because it will produce physiologically identical silk dope that will have undergone the exact post processing, packaging, and excretion systems that native silk does. In addition, it will be 100% pure silk dope, removing the need for any costly or time-consuming purification processes. This will allow the dope to be directly spun into fibers moments after its production. By creating an artificial gland using silk gland cells we will hopefully be able to rapidly produce native spider silk on an industrial scale. Using natural amino acid precursors we will be able to produce a natural, carbon neutral, strong, flexible, biopolymer with a low environmental impact.