Spider silk production using a controlled laboratory system

使用受控实验室系统生产蜘蛛丝

• 批准号: 1945017
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945017
• 关键词: Spider; silk; production; using; controlled

In my project, I will attempt to create a system for producing spider silk that will hopefully provide a better and more reliable method of industrial production. The system I propose will be to dissect out the individual silk glands from the abdomens of orb-weaving species from the Nottingham area and maintain their glands in culture. This ex-vivo system will allow me to more accurately study the mechanisms of cellular silk production. Once this has been achieved I will attempt to create cell lines from the silk glands and use the native protein machinery to produce native silk under laboratory conditions. This system will address the problems faced by previous attempts because it will produce physiologically identical silk dope that will have undergone the exact post processing, packaging, and excretion systems that native silk does. In addition, it will be 100% pure silk dope, removing the need for any costly or time-consuming purification processes. This will allow the dope to be directly spun into fibers moments after its production. By creating an artificial gland using silk gland cells we will hopefully be able to rapidly produce native spider silk on an industrial scale. Using natural amino acid precursors we will be able to produce a natural, carbon neutral, strong, flexible, biopolymer with a low environmental impact.

在我的项目中，我将尝试创建一个生产蜘蛛丝的系统，希望能提供更好、更可靠的工业生产方法。我所建议的系统将是从诺丁汉地区的圆织物种的蛹中解剖出单个的丝腺，并将它们的腺体保存在培养物中。这个离体系统将使我能够更准确地研究细胞丝产生的机制。一旦这一点得到实现，我将尝试从丝腺中建立细胞系，并在实验室条件下使用天然蛋白质机制生产天然丝。该系统将解决先前尝试所面临的问题，因为它将产生生理上相同的丝原液，其将经历天然丝所经历的精确后处理、包装和排泄系统。此外，它将是100%纯丝绸涂料，消除了任何昂贵或耗时的纯化过程的需要。这将允许纺丝原液在其生产后立即直接纺成纤维。通过使用丝腺细胞制造人工腺体，我们将有望在工业规模上快速生产天然蜘蛛丝。使用天然氨基酸前体，我们将能够生产一种天然的、碳中性的、坚固的、灵活的、对环境影响小的生物聚合物。