SBIR Phase II: Targeted Production of Spider Silk Fibroins in Plant Trichomes

SBIR 第二阶段：在植物毛状体中定向生产蜘蛛丝素蛋白

• 批准号: 1230075
• 负责人: Ryan Shepherd
• 金额: $ 49.99万
• 依托单位: PhylloTech Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2015-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1230075&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Targeted; Production

This Small Business Innovation Research (SBIR) Phase II project proposes to develop a platform for the production of spider silk fibroins in plant trichomes. Synthetic spider silk has great potential as an environmentally-friendly biomaterial because it is very flexible yet has a tensile strength greater than steel. A great need exists for a scalable strategy for spider silk fibroin production in a renewable heterologous system that will allow efficient harvest and recovery. The platform is a natural mechanism for protein production and purification in the plant Nicotiana tabacum. The technological innovation is the targeted expression of spider silk fibroins within the gland cells of leaf surface structures called glandular secreting trichomes. The research objectives will be to optimize the production of spider silk fibroins and generate fibers from harvested proteins. The goal is to drive the expression of different spider silk fibroins in trichomes using patented promoters, and refine methods of fibroin recovery, so that fibers with differing physical properties can be generated and tested. This work will demonstrate further that spider silk fibroins can be produced by plant trichomes in a cost-effective manner, and will allow the large-scale, photosynthetic production of next-generation biomaterials.What are the broader impacts of the proposed activity?The broader impact/commercial potential of this project, if successful, will be to demonstrate that a variety of synthetic spider silk fibroins can be produced in a plant-based system, and to better understanding the biosynthetic hardware of the specialized plant structures called glandular secreting trichomes. This work will allow the development of novel ways of achieving industrial biomaterial production without the use of toxic chemicals, and will thus have great societal impact by decreasing the necessity of utilizing large amounts of hazardous compounds in industrial processes. The commercial impact of this project will implement a platform for the photosynthetic production of environmentally-friendly biomaterials.

这个小企业创新研究（SBIR）第二阶段项目建议开发一个平台，用于在植物毛状体中生产蜘蛛丝素蛋白。 合成蜘蛛丝作为一种环境友好的生物材料具有巨大的潜力，因为它非常灵活，但拉伸强度大于钢。 非常需要在可再生的异源系统中生产蜘蛛丝纤蛋白的可扩展策略，其将允许有效的收获和回收。 该平台是烟草植物中蛋白质生产和纯化的天然机制。 这项技术创新是在称为腺分泌毛状体的叶表面结构的腺细胞内有针对性地表达蜘蛛丝丝素蛋白。 研究目标将是优化蜘蛛丝素蛋白的生产，并从收获的蛋白质中产生纤维。 其目标是使用专利启动子驱动不同蜘蛛丝丝素蛋白在毛状体中的表达，并改进丝素蛋白回收方法，从而可以生成和测试具有不同物理特性的纤维。 这项工作将进一步证明蜘蛛丝丝素蛋白可以通过植物毛状体以具有成本效益的方式生产，并将允许大规模光合生产下一代生物材料。拟议活动的广泛影响是什么？该项目的更广泛的影响/商业潜力，如果成功的话，将证明可以在基于植物的系统中生产各种合成蜘蛛丝素蛋白，并更好地了解称为腺体分泌毛状体的专门植物结构的生物合成硬件。 这项工作将允许开发在不使用有毒化学品的情况下实现工业生物材料生产的新方法，从而通过减少在工业过程中使用大量危险化合物的必要性而产生巨大的社会影响。 该项目的商业影响将为光合生产环境友好的生物材料提供平台。