Heterologous production and characterisation of lichen-derived secondary metabolites.

地衣次级代谢产物的异源产生和表征。

• 批准号: 1945087
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1945087
• 关键词: Heterologous; production; characterisation; lichen; derived

Lichen-forming fungi produce a diverse range of unique secondary metabolites possessing a variety of biological activities. Therefore, these natural products have potential for biotechnological and industrial applications. However, the extremely slow growth rates associated with lichens hampers their biotechnological exploitation. Two strategies will be applied to overcome this problem. The first approach will focus on the investigation of the growth rate of lichens. This will involve UV mutagenesis of lichen material in order to create faster-growing mutants. Once such mutants have been generated they will be analysed at the genomic level to obtain insights on the underlying mechanism of growth restriction of the mycobiont. The second approach focuses on the heterologous production and characterisation of secondary metabolites of lichen-derived metabolites. By using bioinformatics and synthetic biology approaches, genes from lichen secondary metabolite biosynthesis gene clusters will be identified and assembled in expression plasmids. These plasmids will be used for transformation of fungal heterologous expression platform strains. Subsequently, metabolite production will be induced and compounds will be tested for their biological activities.

地衣形成真菌产生多种多样的独特的次级代谢产物，具有多种生物活性。因此，这些天然产物具有生物技术和工业应用的潜力。然而，与地衣相关的极低的生长率阻碍了它们的生物技术开发。我们将采取两种策略来解决这个问题。第一种方法将侧重于调查地衣的生长速度。这将涉及地衣材料的紫外线诱变，以产生生长更快的突变体。一旦产生了这样的突变体，将在基因组水平上对其进行分析，以了解真菌生长限制的潜在机制。第二种方法侧重于地衣衍生代谢物的次级代谢物的异源生产和表征。利用生物信息学和合成生物学方法，将地衣次生代谢物生物合成基因簇中的基因鉴定并组装到表达质粒中。这些质粒将用于真菌异源表达平台菌株的转化。随后，将诱导代谢产物产生，并测试化合物的生物活性。

项目成果

Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides.

• DOI: 10.1186/s40694-022-00138-7
• 发表时间: 2022-04-27
• 期刊: Fungal biology and biotechnology