Diagnosis and Pathophysiology Of Pheochromocytoma

嗜铬细胞瘤的诊断和病理生理学

基本信息

项目摘要

Pheochromocytomas are rare but clinically important chromaffin cell tumors that constitute a surgically correctable cause of chronic hypertension. The clinical features and consequences of pheochromocytoma result from release of catecholamines (e.g., norepinephrine and epinephrine) by the tumor. In our protocol studies we would like to find out whether the measurement of plasma metanephrines is the most sensitive biochemical test to diagnose the tumor and whether fluorodopamine positron emission tomography (PET) scanning will improve our ability to localize a pheochromocytoma. In addition, we wish to find out if there are any specific genetic or other markers to predict the course, malignant potential, and recurrence of pheochromocytoma. To date forty patients with known or clinically suspected pheochromocytoma underwent PET scanning after i.v. injection of 6-[18F]fluorodopamine. [18F]fluorodopamine localized the tumor in 28 patients. All patients with normal plasma levels of metanephrines had negative 6-[18F]fluorodopamine PET scans. This concludes that 6-[18F]Fluorodopamine PET scanning can detect and localize pheochromocytomas in patients known to harbor the tumor. Patients in whom the tumor is considered but excluded because of negative plasma metanephrine results have negative 6-[18F]fluorodopamine PET scans. These preliminary findings justify 6-[18F]fluorodopamine PET scanning as a diagnostic tool. In our studies of biochemical diagnosis to date, plasma metanephrines had a sensitivity of 99% and specificity of 90%. The new cloidine test coupled with the measurement of plasma metanephrines is the most promising test to rule out pheochromocytoma in patients presenting with symptoms that resemble the presence of the tumor. Inherited mutations of the RET protooncogene are tumorigenic in patients with multiple endocrine neoplasia type 2. However, it is not understood why only a few of the affected cells in the target organs develop into tumors. Genetic analysis of 9 pheochromocytomas from 5 unrelated patients with MEN 2 showed either duplication of the mutant RET allele in trisomy 10 or loss of the wild-type RET allele. Through either duplication of the mutant allele or loss of the wild-type allele, our results suggest a "second hit" causing a dominant effect of the mutant RET allele as a possible mechanism for pheochromocytoma tumorigenesis in patients with MEN 2. Finally, our laboratory is currently attempting to establish pheochromocytoma cell cultures and used new techniques such as microarray analysis to trace back phenotypic differences in tumors to underlying differences in gene expression and ultimately to the basic somatic or germline mutations responsible for the tumor.
嗜铬细胞瘤是一种罕见但临床上重要的嗜铬细胞肿瘤,它是慢性高血压的一个可手术矫正的病因。嗜铬细胞瘤的临床特征和后果是由儿茶酚胺的释放引起的(例如,去甲肾上腺素和肾上腺素)。在我们的方案研究中,我们想知道血浆变肾上腺素的测量是否是诊断肿瘤的最敏感的生化测试,以及氟多巴正电子发射断层扫描(PET)是否会提高我们定位嗜铬细胞瘤的能力。此外,我们希望找出是否有任何特定的遗传或其他标记来预测嗜铬细胞瘤的过程中,恶性潜力,复发。迄今为止,40例已知或临床疑似嗜铬细胞瘤的患者在静脉注射6-[18F]氟多巴后进行了PET扫描。[18F]氟多巴定位了28例患者的肿瘤。所有血浆变肾上腺素水平正常的患者的6-[18F]氟多巴PET扫描均为阴性。由此得出结论,6-[18F]氟多巴PET扫描可以检测和定位已知患有肿瘤的患者中的嗜铬细胞瘤。考虑肿瘤但因血浆代谢产物结果阴性而被排除的患者,6-[18 F]氟多巴PET扫描结果为阴性。这些初步发现证明6-[18F]氟多巴PET扫描作为诊断工具是合理的。在我们迄今为止的生化诊断研究中,血浆变肾上腺素的敏感性为99%,特异性为90%。新的cloidine测试加上血浆metanephrines的测量是最有希望的测试,以排除嗜铬细胞瘤的患者表现出类似于肿瘤的存在症状。RET原癌基因的遗传性突变在多发性内分泌腺瘤病2型患者中具有致瘤性。然而,目前还不清楚为什么靶器官中只有少数受影响的细胞发展成肿瘤。对来自5名不相关MEN 2患者的9例嗜铬细胞瘤的遗传分析显示,要么在10三体中重复突变RET等位基因,要么丢失野生型RET等位基因。通过突变等位基因的重复或野生型等位基因的丢失,我们的研究结果表明,“二次打击”导致突变RET等位基因的显性效应,作为MEN 2患者嗜铬细胞瘤肿瘤发生的可能机制。最后,我们的实验室目前正试图建立嗜铬细胞瘤细胞培养,并使用新技术,如微阵列分析,以追溯肿瘤的表型差异,潜在的基因表达差异,并最终到负责肿瘤的基本体细胞或种系突变。

项目成果

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Karel Pacak其他文献

Karel Pacak的其他文献

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{{ truncateString('Karel Pacak', 18)}}的其他基金

131I-Metaiodobenzylguanidine Therapy of Pheochromocytoma
131I-间碘苄胍治疗嗜铬细胞瘤
  • 批准号:
    6813962
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
131-I-Metaiodobenzylguanidine Treatment of Malignant Phe
131-I-间碘苄基胍治疗恶性 Phe
  • 批准号:
    7334117
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Diagnosis, Pathophysiology And Molecular Biology Of Pheochromocytoma
嗜铬细胞瘤的诊断、病理生理学和分子生物学
  • 批准号:
    7734764
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Diagnosis, Pathophysiology And Molecular Biology Of Pheo
Pheo 的诊断、病理生理学和分子生物学
  • 批准号:
    7209915
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
New Imaging Modalities In The Diagnosis Of Cushing's
库欣氏病诊断的新成像方式
  • 批准号:
    6813956
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
131-I-Metaiodobenzylguanidine Treatment of Malignant Phe
131-I-间碘苄基胍治疗恶性 Phe
  • 批准号:
    7006751
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
New Imaging Modalities In The Evaluation Of Patients Wit
评估患者机智的新成像方式
  • 批准号:
    6659606
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Diagnosis, Pathophysiology And Molecular Biology Of Pheochromocytoma
嗜铬细胞瘤的诊断、病理生理学和分子生物学
  • 批准号:
    8553901
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Diagnosis, Pathophysiology And Molecular Biology of Pheochromocytoma and Paraganglioma
嗜铬细胞瘤和副神经节瘤的诊断、病理生理学和分子生物学
  • 批准号:
    9339254
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Diagnosis, Pathophysiology And Molecular Biology of Pheochromocytoma and Paraganglioma
嗜铬细胞瘤和副神经节瘤的诊断、病理生理学和分子生物学
  • 批准号:
    10685192
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

相似海外基金

2023 Catecholamines Gordon Research Conference and Gordon Research Seminar
2023年儿茶酚胺戈登研究会议暨戈登研究研讨会
  • 批准号:
    10753909
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
Role of cortical catecholamines in regulating motivated behavior and striatal dopamine
皮质儿茶酚胺在调节动机行为和纹状体多巴胺中的作用
  • 批准号:
    10659716
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
Suppression of LPS-induced TNF alpha production in macrophages by catecholamines is mediated through the beta2-aderenergic receptor
儿茶酚胺通过 β2-肾上腺素能受体介导抑制 LPS 诱导的巨噬细胞中 TNF α 的产生
  • 批准号:
    21K20999
  • 财政年份:
    2021
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Peripheral catecholamines and glucose regulation
外周儿茶酚胺和血糖调节
  • 批准号:
    541159-2019
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
    University Undergraduate Student Research Awards
The roles of glucocorticoids and catecholamines for the testis and peritubular cells
糖皮质激素和儿茶酚胺对睾丸和管周细胞的作用
  • 批准号:
    427588170
  • 财政年份:
    2019
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    --
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    Research Grants
The role of catecholamines in immunotoxicity and tumor response of adoptive T cell therapy in cancer
儿茶酚胺在癌症过继性 T 细胞疗法的免疫毒性和肿瘤反应中的作用
  • 批准号:
    10206054
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
Establishment of nursing care based on scientific knowledge of skin lesions induced by extravasation of catecholamines: an experimental study
基于儿茶酚胺外渗性皮损科学知识的护理建立:实验研究
  • 批准号:
    18K17466
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Peripheral catecholamines and glucose regulation
外周儿茶酚胺和血糖调节
  • 批准号:
    527385-2018
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    University Undergraduate Student Research Awards
The role of catecholamines in immunotoxicity and tumor response of adoptive T cell therapy in cancer
儿茶酚胺在癌症过继性 T 细胞疗法的免疫毒性和肿瘤反应中的作用
  • 批准号:
    10434895
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
Development of fluorescence imaging technology for central catecholamines
中心儿茶酚胺荧光成像技术的进展
  • 批准号:
    17H04029
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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