MULTI PHOTON EXCITATION OF GREEN FLUORESCENT PROTEINS

绿色荧光蛋白的多光子激发

• 批准号: 6444714
• 负责人: Joseph R. LAKOWICZ
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444714
• 关键词: biological products; biomedical equipment development; biomedical resource; informatics; spectrometry; technology /technique

Green fluorescent proteins has become widely used to study gene expression, in cells and even in whole mammalian organisms. Two-Photon induced fluorescence of Green Fluorescence Protein (GFP) mutant was observed and characterized. Unexpectedly we have found that two-photon cross-section of this GFP mutant is enormously high, several time higher than for wild type GFP. The lifetime and anisotropy decays were measured with one and two photon excitation and analyzed globally. The zero time anisotropy for two photon excitation is large than for one photon excitation by the factor 10/7.

绿色荧光蛋白已广泛用于研究基因 在细胞中甚至在整个哺乳动物有机体中表达。 绿色荧光蛋白 (GFP) 的双光子诱导荧光 观察并表征了突变体。 没想到我们发现了 该 GFP 突变体的双光子横截面非常高， 比野生型 GFP 高数倍。 一生和 各向异性衰减通过一个和两个光子激发来测量 进行全球分析。 双光子激发的零时间各向异性 比单光子激发大 10/7 倍。