DNA probes containing solvatochromic fluorescent tags

含有溶剂化显色荧光标签的 DNA 探针

• 批准号: 1947182
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1947182
• 关键词: DNA; probes; containing; solvatochromic; fluorescent

I wish to be attached to the Tucker group, where I undertook my masters project. The group's research comprises of modified DNA and DNA sensing. The group have developed an anthracene DNA sensor for the detection of SNPs. Following on from this work, the project of interest to me consists of developing naphthalimide tagged DNA as a sensor to detect cancer cells. The sensor will be quantitative, detecting the percentage of changed bases, which is important for determining whether the cell is cancerous. Fluorescence will be monitored by UV absorption and TM studies, techniques which I am familiar with after performing them throughout my masters project. These sensors have exciting possibilities to be used clinically, removing cancerous tissue during operations. The cancerous tissue would fluoresce allowing easy excision from the surrounding healthy tissue.

我希望加入塔克小组，在那里我完成了我的硕士项目。该小组的研究包括修饰DNA和DNA传感。该小组开发了一种蒽DNA传感器，用于检测SNPs。在这项工作之后，我感兴趣的项目包括开发萘二甲酰亚胺标记的DNA作为检测癌细胞的传感器。传感器将是定量的，检测变化的碱基的百分比，这对于确定细胞是否是癌性的很重要。荧光将通过紫外吸收和TM研究进行监测，这些技术我在整个硕士项目中进行过之后非常熟悉。这些传感器具有令人兴奋的临床应用可能性，在手术过程中去除癌组织。癌组织会发出荧光，从而可以很容易地从周围的健康组织中切除。