Photon Upconversion Probes with Lanthanide Containing Biomolecules

具有含镧系元素生物分子的光子上转换探针

• 批准号: 2282620
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2282620
• 关键词: Photon; Upconversion; Probes; Lanthanide; Containing

Upconversion of near infra-red light to visible light mediated by lanthanide ions is rapidly emerging as an important phenomenon with many applications in biological sciences, including imaging and medicine, amongst others. Using a single or a combination of different metal ions, lanthanides are capable of converting near infra-red (nIR) light into visible light using low powered lasers and offer much promise over conventional light sources in imaging since biological tissue and materials are much more transparent to radiation in this optical window and the mechanism allows high resolution 3D images to be obtained. However, current upconverting systems are limited to solid-state materials, and are upconverting phosphors or are self assembled supramolecular discrete metal clusters that are kinetically labile and only operate in deuterated solvents. At the interface of chemistry, biology and physics, this project will involve fabrication of a range of biomolecules (proteins, enzymes, peptides) and mimics that are capable of coordinating one or several lanthanide ions in close proximity to one another or a nIR dye antenna to create a new class of upconverting molecule that operates in water for use in biological probe development, biosensing and imaging applications.Specific aims of the project are: To screen a variety of lanthanide and nIR dye systems for upconversion in small polyaminoacrboxylate molecules using statistical methods and then orthogonal protection synthetic methodsTo synthesise/engineer suitable deuterated and non-deuterated peptides, enzymes and proteins incorporating metal specific binding domains and suitable acceptor FRET dyes beginning with Calmodulin and LanmodulinTo characterise the photophysics of these systems using state of the art and time-dependent experimentsTo test the potential of the synthesised upconverting biomolecules for biosensing and imaging applications

由稀土离子介导的近红外光到可见光的上转换正迅速成为一种重要的现象，在生物科学中有许多应用，包括成像和医学等。使用单一或不同金属离子的组合，稀土元素能够使用低功率激光将近红外(NIR)光转换为可见光，并在成像方面提供了比传统光源更有希望的前景，因为生物组织和材料对这种光学窗口中的辐射更加透明，而且这种机制允许获得高分辨率的3D图像。然而，当前的上转换系统仅限于固态材料，并且是上转换荧光粉或自组装的超分子离散金属团簇，其动力学不稳定，只能在氢化溶剂中工作。在化学、生物和物理的交界处，该项目将涉及制造一系列生物分子(蛋白质、酶、肽)和仿制品，这些生物分子能够协调一个或几个紧邻彼此的稀土离子或近红外染料天线，以创建一种新的上转换分子，该分子在水中运行，用于生物探针开发、生物传感和成像应用。该项目的具体目标是：利用统计方法筛选用于上转换的各种稀土和近红外染料体系，以在聚氨基羧酸盐小分子中进行上转换，然后采用正交保护合成方法合成/工程合适的氚和非氚化肽，含有金属特定结合结构域的酶和蛋白质，以及以钙调蛋白和蓝调素开始的合适受体FRET染料使用最先进的和时间相关的实验来表征这些系统的光物理特性测试合成的上转换生物分子用于生物传感和成像应用的潜力